Stricher Mathilde, Vigneron Pascale, Delbecq Frederic, Sarde Claude-Olivier, Egles Christophe
Université de Technologie de Compiègne, CNRS, Biomechanics and Bioengineering, Centre de Recherche Royallieu, CEDEX CS 60 319, 60 203, Compiègne, France.
Université de Technologie de Compiègne, ESCOM, TIMR (Integrated Transformations of Renewable Matter), Centre de Recherche Royallieu, CEDEX CS 60 319, 60 203, Compiègne, France.
Mater Today Bio. 2024 Feb 29;25:101013. doi: 10.1016/j.mtbio.2024.101013. eCollection 2024 Apr.
constitutes, in its most simplified form, a cellularized spheroid built around and stabilised by a form of primitive extracellular matrix (ECM).
We developed a modular approach to soft tissue engineering, by compact stacking V. -based building blocks. This approach is made possible by the structure and cell adhesive properties of these building blocks, which results from the composition of their algal ECM.
A primary biocompatibility assessment demonstrated the cytocompatibility of the algal suspension, its histogenesis-promoting properties, and that it did not induce an inflammatory response . These results allowed us to consider the use of this algal suspension for soft tissue augmentation, and to initiate an biocompatibility study. exhibited cellular fate-directing properties, causing (i) fibroblasts to take on an alkaline phosphatase stem-cell-like phenotype and (ii) both human adipose-derived stem cells and mouse embryonic stem cells to differentiate into preadipocytes to adipocytes. The ability of to support histogenesis and adipogenesis was also observed by subcutaneous tissue augmentation of athymic mice, highlighting the potential of to support or influence tissue regeneration.
We present for the first time as an innovative and inspiring biomaterial for tissue engineering and soft tissue regeneration. Its strategies in terms of shape, structure and composition can be central in the design of a new generation of bio-inspired heterogeneous biomaterials recapitulating more appropriately the complexity of body tissues when guiding their regeneration.
以其最简化的形式而言，是围绕一种原始细胞外基质（ECM）构建并由其稳定的细胞化球体。
我们通过紧密堆叠基于V.的构建模块，开发了一种用于软组织工程的模块化方法。这种方法因这些构建模块的结构和细胞黏附特性而成为可能，这些特性源于其藻类ECM的组成。
初步生物相容性评估证明了藻类悬浮液的细胞相容性、其促进组织发生的特性，并且它不会引发炎症反应。这些结果使我们能够考虑将这种藻类悬浮液用于软组织增强，并启动一项生物相容性研究。表现出细胞命运导向特性，导致（i）成纤维细胞呈现碱性磷酸酶干细胞样表型，以及（ii）人类脂肪来源干细胞和小鼠胚胎干细胞都分化为前脂肪细胞再到脂肪细胞。通过无胸腺小鼠的皮下组织增强也观察到支持组织发生和脂肪生成的能力，突出了支持或影响组织再生的潜力。
我们首次展示了作为用于组织工程和软组织再生的一种创新且鼓舞人心的生物材料。其在形状、结构和组成方面的策略在设计新一代仿生异质生物材料时可能至关重要，这些材料在引导身体组织再生时能更恰当地概括其复杂性。
