School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai, 200237, China.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun, 130012, China.
Environ Sci Pollut Res Int. 2024 Apr;31(17):25202-25215. doi: 10.1007/s11356-024-32420-z. Epub 2024 Mar 11.
Laccase immobilized and cross-linked on FeS/earthworm-like mesoporous SiO (FeS/EW-mSiO) was used to degrade methoxychlor (MXC) in aqueous environments. The effects of various parameters on the degradation of MXC were determined using free and immobilized laccase. Immobilization improved the thermal stability and reuse of laccase significantly. Under the conditions of pH 4.5, temperature 40 °C, and reaction time 8 h, the degradation rate of MXC by immobilized laccase reached a maximum value of 40.99% and remained at 1/3 of the original after six cycles. The excellent degradation performance of FeS/EW-mSiO was attributable to the pyrite (FeS) impurity in FeS, which could act as an electron donor in reductive dehalogenation. Sulfide groups and Fe reduced the activation energy of the system resulting in pyrite-assisted degradation of MXC. The degradation mechanism of MXC in aqueous environments by laccase immobilized on FeS/EW-mSiO was determined via mass spectroscopy of the degradation products. This study is a new attempt to use pyrite to support immobilized laccase degradation.
固定化和交联在 FeS/蚯蚓状介孔硅 (FeS/EW-mSiO) 上的漆酶用于降解水环境中的甲氧氯。使用游离和固定化漆酶确定了各种参数对 MXC 降解的影响。固定化显著提高了漆酶的热稳定性和重复使用性。在 pH 值 4.5、温度 40°C 和反应时间 8 h 的条件下，固定化漆酶对 MXC 的降解率达到最大值 40.99%，经过六次循环后仍保持为原始值的 1/3。FeS/EW-mSiO 的优异降解性能归因于 FeS 中的黄铁矿 (FeS) 杂质，它可以作为还原脱卤反应中的电子供体。硫离子和 Fe 降低了体系的活化能，从而促进了 MXC 的黄铁矿辅助降解。通过降解产物的质谱分析，确定了固定化漆酶在 FeS/EW-mSiO 上在水环境中降解 MXC 的降解机制。这项研究是利用黄铁矿来支持固定化漆酶降解的新尝试。
