Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, 14812, Saudi Arabia.
Bioanalysis. 2024;16(9):401-413. doi: 10.4155/bio-2023-0185. Epub 2024 Mar 11.
Validate a method to quantify 1-(5-fluoropentyl)-N-(2-phenylpropan-2-yl)-1H-indole-3-carboxamide (5F-CUMYL-PICA) and methyl 2-[[1-(5-fluoropentyl) indole-3-carbonyl] amino]-3,3-dimethyl-butanoate (5F-MDMB-PICA) in blood samples using GC-MS/MS. A solid-phase extraction (SPE) method has been developed to quantify 5F-MDMB-PICA and 5F-CUMYL-PICA in authentic human blood samples. The limit of detection (LOD) was 0.1 and 0.11 ng/ml for 5F-CUMYL-PICA and 5F-MDMB-PICA, respectively, while the limit of quantification (LOQ) was 0.50 ng/ml for both two compounds. Recovery was 91.40, 82.54 and 85.10% for SPE, supported liquid extraction (SLE) and ISOLUTE C18; matrix effects 15, 24 and 22.5% for SPE, SLE and ISOLUTE C18; accuracy was 2.4-5.5 and 3.9-7.3% for SPE, SLE and ISOLUTE C18, while precision was 4.6-7.7 and 6.4-8.3% for SPE, SLE and ISOLUTE C18, respectively. The concentrations of 5F-CUMYL-PICA and 5F-MDMB-PICA in the authentic human blood samples were 2.18 and 3.07 ng/ml, respectively. The validated method was successfully used in supporting the quantification of analytes in blood.
验证一种使用 GC-MS/MS 定量分析血液样本中 1-(5-氟戊基)-N-(2-苯基-2-丙基)-1H-吲哚-3-甲酰胺（5F-CUMYL-PICA）和甲基 2-[[1-(5-氟戊基)吲哚-3-羰基]氨基]-3,3-二甲基-丁酸酯（5F-MDMB-PICA）的方法。已经开发了一种固相萃取（SPE）方法来定量分析真实人体血液样本中的 5F-MDMB-PICA 和 5F-CUMYL-PICA。5F-CUMYL-PICA 和 5F-MDMB-PICA 的检测限（LOD）分别为 0.1 和 0.11ng/ml，定量限（LOQ）分别为 0.50ng/ml。SPE、支持液提取（SLE）和 ISOLUTE C18 的回收率分别为 91.40%、82.54%和 85.10%；SPE、SLE 和 ISOLUTE C18 的基质效应分别为 15%、24%和 22.5%；SPE、SLE 和 ISOLUTE C18 的准确度分别为 2.4%-5.5%、3.9%-7.3%；SPE、SLE 和 ISOLUTE C18 的精密度分别为 4.6%-7.7%、6.4%-8.3%。真实人体血液样本中 5F-CUMYL-PICA 和 5F-MDMB-PICA 的浓度分别为 2.18ng/ml 和 3.07ng/ml。该验证方法成功用于支持血液中分析物的定量分析。
