Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ 28013-602, Brazil; Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Brazil.
Laboratório de Biologia Celular e Tecidual, CBB, UENF, Brazil.
J Proteomics. 2024 May 15;299:105156. doi: 10.1016/j.jprot.2024.105156. Epub 2024 Mar 11.
Plants exhibit phenotypic plasticity in response to environmental variations, which can lead to stable genetic and physiological adaptations if exposure to specific conditions is prolonged. Myrsine coriacea demonstrates this through its ability to thrive in diverse environments. The objective of the article is to investigate potential differences in protein accumulation and physiological responses of M. coriacea by cultivating plants from seeds collected from four populations at different altitudes in a common garden experiment. Additionally, we aim to evaluate whether these differences exhibit genetic fixation. Through integrated physiological and proteomic analyses, we identified 170 differentially accumulated proteins and observed significant physiological differences among the populations. The high-altitude population (POP1) exhibited a unique proteomic profile with significant down-regulation of proteins involved in carbon fixation and energy metabolism, suggesting a potential reduction in photosynthetic efficiency. Physiological analyses showed lower leaf nitrogen content, net CO assimilation rate, specific leaf area, and relative growth rate in stem height for POP1, alongside higher leaf carbon isotopic composition (δ13C) and leaf carbon (C) content. These findings provide insight into the complex interplay between proteomic and physiological adaptations in M. coriacea and underscore the importance of local adaptations. SIGNIFICANCE: We investigate the adaptive responses of M. coriacea, a shrub with a broad phenotypic range, by cultivating plants from seeds collected at four different altitudes in a common garden experiment. These findings provide insight into the complex interplay between proteomic and physiological adaptations in M. coriacea and underscore the importance of local adaptations in the face of climate change. This study contributes to advancing our understanding of the influence of altitude-specific selection pressures on the molecular biology and physiology of plants in natural populations. Our findings provide valuable insights that enhance our ability to predict and comprehend how plants respond to climate change.
植物会对环境变化表现出表型可塑性，如果暴露于特定条件的时间延长，这可能会导致稳定的遗传和生理适应。Myrsine coriacea 就是通过在不同环境中茁壮成长来展示这种能力的。本文的目的是通过在一个共同的花园实验中，从四个不同海拔的种群中收集的种子来培养植物，研究 M. coriacea 的蛋白质积累和生理反应的潜在差异。此外，我们旨在评估这些差异是否表现出遗传固定。通过综合生理和蛋白质组学分析，我们鉴定了 170 个差异积累蛋白，并观察到种群之间存在显著的生理差异。高海拔种群 (POP1) 表现出独特的蛋白质组学特征，与碳固定和能量代谢相关的蛋白质显著下调，表明潜在的光合作用效率降低。生理分析显示，POP1 的叶片氮含量、净 CO 同化率、比叶面积和茎高的相对生长率较低，同时叶片碳同位素组成 (δ13C) 和叶片碳 (C) 含量较高。这些发现深入了解了 M. coriacea 中蛋白质组学和生理适应之间的复杂相互作用，并强调了局部适应的重要性。意义：我们通过在一个共同的花园实验中，从四个不同海拔的种群中收集的种子来培养植物，研究了具有广泛表型范围的灌木 M. coriacea 的适应反应。这些发现深入了解了 M. coriacea 中蛋白质组学和生理适应之间的复杂相互作用，并强调了局部适应在应对气候变化时的重要性。本研究有助于推进我们对特定海拔选择压力对自然种群中植物分子生物学和生理学影响的理解。我们的发现提供了有价值的见解，增强了我们预测和理解植物对气候变化反应的能力。
