Minello Luana Vanessa Peretti, Kuntzler Suelen Goettems, Lamb Thainá Inês, Neves Cleo de Oliveira, Berghahn Emílio, da Paschoa Roberta Pena, Silveira Vanildo, de Lima Jeferson Camargo, Aguzzoli Cesar, Sperotto Raul Antonio
Botany Department, Graduate Program in Plant Physiology, Biology Institute, Federal University of Pelotas, Pelotas, Brazil.
Graduate Program in Biotechnology, University of Vale do Taquari - Univates, Lajeado, Brazil.
Front Plant Sci. 2024 Sep 18;15:1422935. doi: 10.3389/fpls.2024.1422935. eCollection 2024.
The use of biofertilizers is becoming an economical and environmentally friendly alternative to promote sustainable agriculture. Biochar from microalgae/cyanobacteria can be applied to enhance the productivity of food crops through soil improvement, slow nutrient absorption and release, increased water uptake, and long-term mitigation of greenhouse gas sequestration. Therefore, the aim of this study was to evaluate the stimulatory effects of biochar produced from Spirulina () biomass on the development and seed production of rice plants. Biochar was produced by slow pyrolysis at 300°C, and characterization was performed through microscopy, chemical, and structural composition analyses. Molecular and physiological analyses were performed in rice plants submitted to different biochar concentrations (0.02, 0.1, and 0.5 mg mL) to assess growth and productivity parameters. Morphological and physicochemical characterization revealed a heterogeneous morphology and the presence of several minerals (Na, K, P, Mg, Ca, S, Fe, and Si) in the biochar composition. Chemical modification of compounds post-pyrolysis and a highly porous structure with micropores were observed. Rice plants submitted to 0.5 mg mL of biochar presented a decrease in root length, followed by an increase in root dry weight. The same concentration influenced seed production, with an increase of 44% in the number of seeds per plant, 17% in the percentage of full seeds per plant, 12% in the weight of 1,000 full seeds, 53% in the seed weight per plant, and 12% in grain area. Differential proteomic analyses in shoots and roots of rice plants submitted to 0.5 mg mL of biochar for 20 days revealed a fine-tuning of resource allocation towards seed production. These results suggest that biochar derived from biomass can stimulate rice seed production.
生物肥料的使用正成为促进可持续农业发展的一种经济且环保的选择。微藻/蓝细菌产生的生物炭可通过改善土壤、缓慢吸收和释放养分、增加水分吸收以及长期缓解温室气体固存来提高粮食作物的产量。因此，本研究的目的是评估螺旋藻生物质产生的生物炭对水稻植株发育和种子生产的刺激作用。生物炭通过在300°C下缓慢热解产生，并通过显微镜、化学和结构组成分析进行表征。对处于不同生物炭浓度（0.02、0.1和0.5 mg/mL）的水稻植株进行分子和生理分析，以评估生长和生产力参数。形态学和物理化学表征揭示了生物炭组成中形态的不均匀性以及几种矿物质（钠、钾、磷、镁、钙、硫、铁和硅）的存在。观察到热解后化合物的化学修饰以及具有微孔的高度多孔结构。施用0.5 mg/mL生物炭的水稻植株根长缩短，随后根干重增加。相同浓度影响种子生产，单株种子数增加44%，单株饱满种子百分比增加17%，1000粒饱满种子重量增加12%，单株种子重量增加53%，粒面积增加12%。对施用0.5 mg/mL生物炭20天的水稻植株地上部和根部进行差异蛋白质组学分析，结果表明资源分配朝着种子生产进行了微调。这些结果表明，螺旋藻生物质衍生的生物炭可以刺激水稻种子生产。
