He Wei, Zhang Zhe, Tan ZiLong, Liu XinXian, Wang ZeKun, Xiong Bo, Shen XiaoLi, Zhu XinGen
Department of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.
Institute of Neuroscience, Nanchang University, Nanchang, Jiangxi, China.
Sci Rep. 2024 Mar 11;14(1):5861. doi: 10.1038/s41598-024-56493-5.
There has been an upward trend in the incidence of glioma, with high recurrence and high mortality. The beta subunits of the 20S proteasome are encoded by the proteasome beta (PSMB) genes and may affect the proteasome's function in glioma, assembly and inhibitor binding. This study attempted to reveal the function of the proliferation and invasion of glioma cells, which is affected by proteasome 20S subunit beta 2 (PSMB2). We subjected the data downloaded from the TCGA database to ROC, survival, and enrichment analyses. After establishing the stable PSMB2 knockdown glioma cell line. We detect the changes in the proliferation, invasion and migration of glioma cells by plate colony formation assay, transwell assay, wound healing assay and flow cytometry and PSMB2 expression was verified by quantitative PCR and Western blotting to identify the mRNA and protein levels. PSMB2 expression was higher in glioma tissues, and its expression positively correlated with poor prognosis and high tumor grade and after PSMB2 knockdown, the proliferation, invasion and migration of glioma cells were weakened.
胶质瘤的发病率呈上升趋势，具有高复发率和高死亡率。20S蛋白酶体的β亚基由蛋白酶体β（PSMB）基因编码，可能影响蛋白酶体在胶质瘤中的功能、组装和抑制剂结合。本研究试图揭示受蛋白酶体20S亚基β2（PSMB2）影响的胶质瘤细胞增殖和侵袭的功能。我们对从TCGA数据库下载的数据进行了ROC、生存和富集分析。在建立稳定的PSMB2敲低胶质瘤细胞系后。我们通过平板集落形成试验、transwell试验、伤口愈合试验和流式细胞术检测胶质瘤细胞增殖、侵袭和迁移的变化，并通过定量PCR和蛋白质印迹法验证PSMB2表达，以确定mRNA和蛋白质水平。PSMB2在胶质瘤组织中表达较高，其表达与预后不良和肿瘤分级高呈正相关，PSMB2敲低后，胶质瘤细胞的增殖、侵袭和迁移减弱。
