Wang Dianyun, Zhang Caiying, Guo Huiling, Cui Ting, Pu Wenjing, Huang Bingyan, Zhu Jiamei, Dai Xueyan
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Biol Trace Elem Res. 2025 Jan;203(1):374-383. doi: 10.1007/s12011-024-04144-1. Epub 2024 Mar 12.
Excessive doses of molybdenum (Mo) and cadmium (Cd) have toxic effects on animals. Nevertheless, the reproductive toxicity elicited by Mo and Cd co-exposure remains obscure. To evaluate the co-induce toxic impacts of Mo and Cd on ovaries, 8-day-old 40 healthy ducks were stochastically distributed to four groups and were raised a basal diet supplemented with Cd (4 mg/kg Cd) and/or Mo (100 mg/kg Mo). In the 16th week, ovary tissues were gathered. The data revealed that Mo and/or Cd decreased GSH content, CAT, T-SOD, and GSH-Px activities and increased MDA and HO levels. Moreover, there was a significant decrease in nuclear Nrf2 protein level and its related downstream factors, while cytoplasmic Nrf2 protein level showed a substantial increase. Additionally, a marked elevation was observed in ferrous ion content and TFRC, GCLC, SLC7A11, ACSL4, and PTGS2 expression levels, while FTH1, FTL1, FPN1, and GPX4 expression levels were conversely reduced. These indicators exhibited more marked changes in the joint exposure group. In brief, our results announced that Mo and/or Cd resulted in oxidative stress and ferroptosis in duck ovaries. Synchronously, the Cd and Mo mixture intensified the impacts.
过量的钼（Mo）和镉（Cd）对动物具有毒性作用。然而，钼和镉共同暴露引起的生殖毒性仍不清楚。为了评估钼和镉对卵巢的共同诱导毒性影响，将8日龄的40只健康鸭子随机分为四组，用添加了镉（4毫克/千克镉）和/或钼（100毫克/千克钼）的基础日粮饲养。在第16周时，采集卵巢组织。数据显示，钼和/或镉降低了谷胱甘肽（GSH）含量、过氧化氢酶（CAT）、总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，并增加了丙二醛（MDA）和血红素加氧酶（HO）水平。此外，核因子E2相关因子2（Nrf2）蛋白水平及其相关下游因子显著降低，而细胞质Nrf2蛋白水平则显著升高。此外，观察到亚铁离子含量以及转铁蛋白受体（TFRC）、谷氨酸半胱氨酸连接酶催化亚基（GCLC）、溶质载体家族7成员11（SLC7A11）、长链脂酰辅酶A合成酶4（ACSL4）和前列腺素内过氧化物合酶2（PTGS2）表达水平显著升高，而铁蛋白重链1（FTH1）、铁蛋白轻链1（FTL1）、铁转运蛋白1（FPN1）和谷胱甘肽过氧化物酶4（GPX4）表达水平则相反降低。这些指标在联合暴露组中表现出更明显的变化。简而言之，我们的结果表明，钼和/或镉导致鸭卵巢氧化应激和铁死亡。同时，镉和钼的混合物加剧了这种影响。
