Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, India.
Department of Pharmacology, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune, India.
Curr Drug Discov Technol. 2025;22(1):e080324227818. doi: 10.2174/0115701638276524240305054259.
Type 1 diabetes mellitus (T1DM) is a condition marked by elevated blood sugar levels and primarily recognized by the destruction of beta cells caused by an autoimmune attack, which is a significant characteristic of T1DM. Recent studies have demonstrated the regenerative potential of conditioned medium therapy. In light of this, the current research sought to assess the impact of Mesenchymal Stem Cell conditioned media (CM) and CM with resveratrol (CM+ Resveratrol) on the management of T1DM in Swiss albino mice. By leveraging and modifying existing conditioned medium therapy, this study aims to evaluate its effectiveness in treating T1DM.
MATERIALS & METHODS: Diabetes was induced in animals using the diabetes-inducing agent streptozotocin (STZ). The animals were then divided into five groups: Normal control, Disease Control, Resveratrol, Condition Media, and CM + Resveratrol. Treatments were given to the animals accordingly. The study period was 28 days. During this time, the animals were monitored for foodwater intake twice a week, blood glucose levels, and body weight. At the conclusion of the 28-day study period, biochemical estimations were performed for serum insulin levels, C-peptide levels, anti-inflammatory cytokines levels and pro-inflammatory cytokines levels. Additionally, histopathology of the pancreas was performed.
The test groups showed a significant decrease in blood glucose levels, an increase in Cpeptide levels, and a decrease in pro-inflammatory cytokine levels compared to the disease group. However, no statistically significant change within groups was observed in terms of serum insulin and anti-inflammatory cytokine levels. The improvement in diabetic symptoms, such as polyphagia, polydipsia, and weight loss, was observed in the treatment group, along with pancreatic regeneration, which indicated improved insulin secretion.
In the current investigation, we concluded that CM and CM+ Resveratrol, as natural immunomodulators, have the capacity to regenerate injured pancreatic beta cells and have antidiabetic action, together with immunomodulating impact. Nonetheless, future studies on this therapy appear to be promising.
1 型糖尿病（T1DM）的特点是血糖升高，主要由自身免疫攻击导致的β细胞破坏引起，这是 T1DM 的一个重要特征。最近的研究表明，条件培养基疗法具有再生潜力。鉴于此，本研究旨在评估间充质干细胞条件培养基（CM）和含白藜芦醇的 CM（CM+Resveratrol）对瑞士白化病小鼠 T1DM 的治疗作用。通过利用和修改现有的条件培养基疗法，本研究旨在评估其治疗 T1DM 的效果。
使用糖尿病诱导剂链脲佐菌素（STZ）诱导动物发生糖尿病。然后将动物分为五组：正常对照组、疾病对照组、白藜芦醇组、条件培养基组和 CM+Resveratrol 组。相应地给予动物治疗。研究期为 28 天。在此期间，每周两次监测动物的食物和水摄入量、血糖水平和体重。在 28 天的研究结束时，进行血清胰岛素水平、C 肽水平、抗炎细胞因子水平和促炎细胞因子水平的生化评估。此外，还进行了胰腺组织病理学检查。
与疾病组相比，实验组的血糖水平显著降低，C 肽水平升高，促炎细胞因子水平降低。然而，在血清胰岛素和抗炎细胞因子水平方面，组内没有观察到统计学上的显著变化。治疗组观察到糖尿病症状（多饮、多食和体重减轻）得到改善，同时胰腺再生，表明胰岛素分泌得到改善。
在本研究中，我们得出结论，CM 和 CM+Resveratrol 作为天然免疫调节剂，具有再生受损的胰岛β细胞的能力，并具有抗糖尿病作用和免疫调节作用。然而，这种治疗方法的进一步研究似乎很有前途。
