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牛脐带间充质干细胞条件培养基增加了烟酰胺-链脲佐菌素诱导的糖尿病大鼠胰腺中 GLUT-4 的表达。

Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin.

机构信息

Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia.

Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

出版信息

Open Vet J. 2024 Aug;14(8):1761-1770. doi: 10.5455/OVJ.2024.v14.i8.3. Epub 2024 Aug 31.

DOI:10.5455/OVJ.2024.v14.i8.3
PMID:39308716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11415927/
Abstract

BACKGROUND

Diabetes is a degenerative disease associated with metabolic disorders. The majority of people have type 2 diabetes mellitus (DM) insulin resistance due to an unhealthy lifestyle. The development of DM treatment is also growing, one of which is using conditioned medium.

AIM

This study aims to determine the effect of Bovine umbilical mesenchymal stem cell-conditioned medium (BUMSC-CM) on nicotinamide (NA) and streptozotocin (STZ) induced rats as an animal model of DM.

METHODS

The study began with the docking of Cholecalciferol with aldolase reductase and glucokinase. In the study, animal models were divided into five groups: group A (negative control), group B (diabetic rats), group C (NA+STZ+Metformin), group D (NA+STZ+ BUMSC-CM 0.2 ml/kg BW), and group E (NA+STZ+ BUMSC-CM 0.5 ml/kg BW). Blood sugar levels were checked, and BUMSC-CM was administered by intramuscular injection at four-day intervals for a duration of 16 days. Blood sugar levels were also sampled, and GLUT4 histochemical and immunohistochemical staining was performed.

RESULTS

The results showed that Cholecalciferol can bind to aldolase reductase ASP43 and TYR48 and bind to glucokinase at TYR214 with hydrogen bonds. BUMSC-CM administration was able to reduce blood sugar well. In addition, BUMSC-CM also helped repair the tissue structure of the pancreas damaged by inflammation from STZ administration.

CONCLUSION

This study can be concluded that the administration of BUMSC-CM can be an alternative cell-free therapy for patients with DM.

摘要

背景

糖尿病是一种与代谢紊乱相关的退行性疾病。大多数人因不健康的生活方式而患有 2 型糖尿病（DM）胰岛素抵抗。DM 治疗的发展也在不断增长，其中之一是使用条件培养基。

目的

本研究旨在确定牛脐带间充质干细胞条件培养基（BUMSC-CM）对烟酰胺（NA）和链脲佐菌素（STZ）诱导的大鼠的影响，作为 DM 的动物模型。

方法

该研究首先进行了胆钙化醇与醛缩酶还原酶和葡萄糖激酶的对接。在研究中，动物模型分为五组：A 组（阴性对照）、B 组（糖尿病大鼠）、C 组（NA+STZ+二甲双胍）、D 组（NA+STZ+BUMSC-CM 0.2 ml/kg BW）和 E 组（NA+STZ+BUMSC-CM 0.5 ml/kg BW）。检查血糖水平，并每隔四天通过肌肉注射给予 BUMSC-CM，持续 16 天。还采集了血糖样本，并进行了 GLUT4 组织化学和免疫组织化学染色。

结果

结果表明，胆钙化醇可以与醛缩酶还原酶 ASP43 和 TYR48 结合，并与葡萄糖激酶在 TYR214 处通过氢键结合。BUMSC-CM 的给药能够很好地降低血糖。此外，BUMSC-CM 还有助于修复 STZ 给药引起的炎症对胰腺组织结构的损伤。

结论

本研究可以得出结论，BUMSC-CM 的给药可以作为 DM 患者的一种无细胞治疗的替代方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/004d839606da/OpenVetJ-14-1761-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/f4359fa812c5/OpenVetJ-14-1761-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/dd8cbc148b52/OpenVetJ-14-1761-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/18012deb2f6f/OpenVetJ-14-1761-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/714dcf7716b0/OpenVetJ-14-1761-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/c2c315a1d057/OpenVetJ-14-1761-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/004d839606da/OpenVetJ-14-1761-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/f4359fa812c5/OpenVetJ-14-1761-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/dd8cbc148b52/OpenVetJ-14-1761-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/18012deb2f6f/OpenVetJ-14-1761-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/714dcf7716b0/OpenVetJ-14-1761-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/c2c315a1d057/OpenVetJ-14-1761-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b99/11415927/004d839606da/OpenVetJ-14-1761-g006.jpg

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本文引用的文献

1
Towards the Standardization of Mesenchymal Stem Cell Secretome-Derived Product Manufacturing for Tissue Regeneration.迈向组织再生用间充质干细胞分泌组衍生产品制造的标准化。
Int J Mol Sci. 2023 Aug 9;24(16):12594. doi: 10.3390/ijms241612594.
2
The evolving view of thermogenic fat and its implications in cancer and metabolic diseases.生热脂肪的不断演变的观点及其在癌症和代谢性疾病中的意义。
Signal Transduct Target Ther. 2022 Sep 16;7(1):324. doi: 10.1038/s41392-022-01178-6.
3
Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model.
去铁胺预处理脐带间充质干细胞条件培养液对糖尿病肾病模型的治疗潜力。
Stem Cell Res Ther. 2022 Sep 2;13(1):438. doi: 10.1186/s13287-022-03121-6.
4
Stem cell-based therapy for human diseases.基于干细胞的人类疾病治疗方法。
Signal Transduct Target Ther. 2022 Aug 6;7(1):272. doi: 10.1038/s41392-022-01134-4.
5
Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling.维生素 D3 通过 SIRT1 信号通路刺激人骨髓间充质基质/干细胞的增殖能力、多能性标志物表达和成骨作用。
Biomolecules. 2022 Feb 18;12(2):323. doi: 10.3390/biom12020323.
6
In Vitro Neuroprotective Effect of the Bovine Umbilical Vein Endothelial Cell Conditioned Medium Mediated by Downregulation of IL-1β, Caspase-3, and Caspase-9 Expression.牛脐静脉内皮细胞条件培养基通过下调IL-1β、Caspase-3和Caspase-9表达介导的体外神经保护作用
Vet Sci. 2022 Jan 27;9(2):48. doi: 10.3390/vetsci9020048.
7
Glucokinase as an emerging anti-diabetes target and recent progress in the development of its agonists.葡萄糖激酶作为一个新兴的抗糖尿病靶点及其激动剂的研发进展。
J Enzyme Inhib Med Chem. 2022 Dec;37(1):606-615. doi: 10.1080/14756366.2021.2025362.
8
Physiological and Pathological Roles of Aldose Reductase.醛糖还原酶的生理和病理作用
Metabolites. 2021 Sep 27;11(10):655. doi: 10.3390/metabo11100655.
9
Diabetes Mellitus and Its Metabolic Complications: The Role of Adipose Tissues.糖尿病及其代谢并发症：脂肪组织的作用。
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Arch Pharm Res. 2021 Jul;44(7):655-667. doi: 10.1007/s12272-021-01343-5. Epub 2021 Jul 19.