Clifton Eric H, Castrillo Louela A, Jaronski Stefan T, Hajek Ann E
Department of Entomology, Cornell University, Ithaca, NY, United States.
Emerging Pests and Pathogens Research, USDA-Agricultural Research Service, Ithaca, NY, United States.
Front Insect Sci. 2023 Apr 25;3:1127682. doi: 10.3389/finsc.2023.1127682. eCollection 2023.
The entomopathogenic fungus is cosmopolitan and known to infect a variety of sap-sucking pests like aphids, mealybugs, and scales in the order of Hemiptera. In Fall 2017, spotted lanternfly (SLF) adults killed by the fungal entomopathogen were found in Berks County, Pennsylvania. In 2018-2020 we collected SLF and nearby non-target insects killed by spp. from 18 field sites in southeastern Pennsylvania. We identified 159 isolates from SLF and six isolates from non-targets. Five isolates of and one isolate of were identified from the non-targets. Based on sequence data from the nuclear B locus (Bloc) intergenic region, all the isolates from SLF were identified as , but there were 20 different strains within this species, grouped into two clades. Three strains (A, B, and L) were found in most field sites and were the most prevalent. Representative isolates for these three strains were used in laboratory bioassays and were compared to a commercial strain (GHA). Strain B was inferior to A, L, and GHA against nymphs; strains A and L had greater efficacy than B and GHA against adults. We also quantified conidial production on SLF cadavers. This paper discusses the diversity of these strains in SLF populations and implications for biological control of this abundant invasive.
昆虫病原真菌分布广泛，已知能感染多种半翅目吸食性害虫，如蚜虫、粉蚧和介壳虫。2017年秋季，在宾夕法尼亚州伯克郡发现了被这种真菌性昆虫病原体杀死的斑点灯笼蝇成虫。在2018 - 2020年期间，我们从宾夕法尼亚州东南部的18个田间地点收集了被该菌杀死的斑点灯笼蝇及附近的非靶标昆虫。我们从斑点灯笼蝇中鉴定出159个该菌分离株，从非靶标昆虫中鉴定出6个分离株。从非靶标昆虫中鉴定出5个该菌分离株和1个另一种菌的分离株。基于核B位点（Bloc）基因间区域的序列数据，所有来自斑点灯笼蝇的分离株都被鉴定为该菌，但该物种内有20种不同的菌株，分为两个进化枝。在大多数田间地点都发现了三种该菌菌株（A、B和L），且最为普遍。这三种菌株的代表性分离株用于实验室生物测定，并与一种商业该菌菌株（GHA）进行比较。菌株B对若虫的防治效果不如A、L和GHA；菌株A和L对成虫的防治效果比B和GHA更好。我们还对斑点灯笼蝇尸体上的分生孢子产量进行了量化。本文讨论了这些该菌菌株在斑点灯笼蝇种群中的多样性以及对这种大量入侵害虫进行生物防治的意义。
