Department of Botany, M.M.V., Banaras Hindu University, Varanasi 221005, India.
Department of Botany, University of Allahabad, Allahabad 211002, India.
J Proteome Res. 2024 Aug 2;23(8):2680-2699. doi: 10.1021/acs.jproteome.3c00779. Epub 2024 Mar 12.
Cyanobacteria (oxygenic photoautrophs) comprise a diverse group holding significance both environmentally and for biotechnological applications. The utilization of proteomic techniques has significantly influenced investigations concerning cyanobacteria. Application of proteomics allows for large-scale analysis of protein expression and function within cyanobacterial systems. The cyanobacterial proteome exhibits tremendous functional, spatial, and temporal diversity regulated by multiple factors that continuously modify protein abundance, post-translational modifications, interactions, localization, and activity to meet the dynamic needs of these tiny blue greens. Modern mass spectrometry-based proteomics techniques enable system-wide examination of proteome complexity through global identification and high-throughput quantification of proteins. These powerful approaches have revolutionized our understanding of proteome dynamics and promise to provide novel insights into integrated cellular behavior at an unprecedented scale. In this Review, we present modern methods and cutting-edge technologies employed for unraveling the spatiotemporal diversity and dynamics of cyanobacterial proteomics with a specific focus on the methods used to analyze post-translational modifications (PTMs) and examples of dynamic changes in the cyanobacterial proteome investigated by proteomic approaches.
蓝细菌（产氧光合自养生物）是一个多样化的群体，它们在环境和生物技术应用方面都具有重要意义。蛋白质组学技术的应用极大地影响了对蓝细菌的研究。蛋白质组学的应用可以在蓝细菌系统中大规模分析蛋白质的表达和功能。蓝细菌的蛋白质组表现出巨大的功能、空间和时间多样性，受多种因素调节，这些因素不断改变蛋白质丰度、翻译后修饰、相互作用、定位和活性，以满足这些微小蓝绿藻的动态需求。基于现代质谱的蛋白质组学技术能够通过对蛋白质进行全局鉴定和高通量定量，对蛋白质组的复杂性进行系统全面的检查。这些强大的方法彻底改变了我们对蛋白质组动力学的理解，并有望以空前的规模提供对细胞整体行为的新见解。在这篇综述中，我们介绍了用于揭示蓝细菌蛋白质组学时空多样性和动力学的现代方法和前沿技术，特别关注用于分析翻译后修饰（PTMs）的方法以及蛋白质组学方法研究的蓝细菌蛋白质组中动态变化的实例。
