1型或3型慢性丙型病毒性肝炎患者血液自然杀伤细胞表型与肝纤维化严重程度的相关性

Association of Blood NK Cell Phenotype with the Severity of Liver Fibrosis in Patients with Chronic Viral Hepatitis C with Genotype 1 or 3.

作者信息

Tsukanov Vladislav Vladimirovich, Savchenko Andrei Anatolyevich, Cherepnin Mikhail Aleksandrovich, Kasparov Eduard Vilyamovich, Tikhonova Elena Petrovna, Vasyutin Alexander Viktorovich, Tonkikh Julia Leongardovna, Anisimova Anna Alexandrovna, Belenyuk Vasily Dmitrievich, Borisov Alexandr Gennadyevich

机构信息

Federal Research Center "Krasnoyarsk Science Center" of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia.

Krasnoyarsk State Medical University Named after Prof. V.F. Voino-Yasenetsky of the Ministry of Healthcare of Russian Federation, 660022 Krasnoyarsk, Russia.

出版信息

Diagnostics (Basel). 2024 Feb 21;14(5):472. doi: 10.3390/diagnostics14050472.

DOI:10.3390/diagnostics14050472

PMID:38472945

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10930504/

Abstract

BACKGROUND

NK cells phenotype and functional state in different genotypes of chronic viral hepatitis C (CVHC), depending on liver fibrosis severity, have not been sufficiently studied, which limits the possibilities for the development of pathology therapy.

METHODS

The CVHC diagnosis was based on the EASL recommendations (2018). Clinical examination with liver elastometry was performed in 297 patients with genotype 1 and in 231 patients with genotype 3 CVHC. The blood NK cells phenotype was determined by flow cytometry in 74 individuals with genotype 1 and in 69 individuals with genotype 3 CVHC.

RESULTS

The frequency of METAVIR liver fibrosis stages F3-F4 was 32.5% in individuals with genotype 3, and 20.5% in individuals with genotype 1 CVHC ( = 0.003). In patients with both genotype 1 and genotype 3 CVHC, a decrease in the total number of blood NK cells, CD56CD16 NK cells and an increase in the proportion of CD56CD16 NK cells, CD94 and CD38 CD73 NK cells were registered in patients with fibrosis stage F3-F4 by METAVIR in comparison with persons with METAVIR fibrosis stage F0-F1.

CONCLUSIONS

In patients with both genotype 1 and genotype 3 CVHC, an imbalance in the ratio between cytokine-producing and cytotoxic NK cells and an increase in the content of NK cells that express inhibitory molecules were determined in patients with severe liver fibrosis.

摘要

背景

不同基因型慢性丙型病毒性肝炎（CVHC）中自然杀伤（NK）细胞的表型和功能状态，取决于肝纤维化的严重程度，目前尚未得到充分研究，这限制了病理治疗发展的可能性。

方法

CVHC的诊断基于欧洲肝脏研究学会（EASL）的建议（2018年）。对297例基因1型和231例基因3型CVHC患者进行了包括肝脏弹性测定的临床检查。通过流式细胞术测定了74例基因1型和69例基因3型CVHC患者血液中NK细胞的表型。

结果

基因3型患者中METAVIR肝纤维化分期F3 - F4的频率为32.5%，基因1型CVHC患者为20.5%（P = 0.003）。与METAVIR纤维化分期F0 - F1的患者相比，基因1型和基因3型CVHC患者中，METAVIR纤维化分期F3 - F4的患者血液中NK细胞总数、CD56CD16 NK细胞减少，而CD56CD16 NK细胞、CD94和CD38+CD73 NK细胞比例增加。