缺血性心脏病患者血液中的生物膜特异性基因以及抗菌和抗炎生物标志物
Biofilm-Specific Genes and Antimicrobial and Anti-Inflammatory Biomarkers in the Blood of Patients with Ischemic Heart Disease.
作者信息
Giedraitiene Agne, Tatarunas Vacis, Kaminskaite Kornelija, Meskauskaite Ugne, Boieva Svitlana, Ajima Yu, Ciapiene Ieva, Veikutiene Audrone, Zvikas Vaidotas, Kupstyte-Kristapone Nora, Jakstas Valdas, Luksiene Dalia, Tamosiunas Abdonas, Lesauskaite Vaiva
机构信息
Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Eiveniu 4, LT 50161 Kaunas, Lithuania.
Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu 15, LT 50103 Kaunas, Lithuania.
出版信息
Diagnostics (Basel). 2024 Mar 5;14(5):546. doi: 10.3390/diagnostics14050546.
BACKGROUND
Ischemic heart disease (IHD) is the most prevalent type of cardiovascular disease. The main cause of IHD is atherosclerosis, which is a multifactorial inflammatory disease of blood vessels. Studies show that bacteria might have a significant impact on the pathogenesis of atherosclerosis and plaque rupture. This study aimed to evaluate the complexity of interactions between bacteria and the human body concerning metabolites and bacterial genes in patients with ischemic heart disease.
METHODS
Bacterial and , , and genes were detected in whole blood using a real-time PCR methodology. An enzyme-linked immunosorbent assay was used to measure the concentration of the LL-37 protein. An analysis of ARA in blood plasma was performed.
RESULTS
Bacterial was detected in 31% of the study patients, and the genes and in 20%. genes were detected more frequently in patients younger than 65 years than in patients aged 65 years and older ( = 0.018) and in patients with type 2 diabetes ( = 0.048). Concentrations of the human antimicrobial peptide LL-37 and 12S-HETE concentrations were determined to be higher if patients had and biofilm-specific genes.
CONCLUSIONS
The results of this study enhance the understanding that bacteria may participate in the pathogenesis of atherosclerosis and IHD. Bacterial DNA and host metabolites in higher concentrations appear to be detected.
背景
缺血性心脏病（IHD）是最常见的心血管疾病类型。IHD的主要病因是动脉粥样硬化，这是一种血管的多因素炎症性疾病。研究表明，细菌可能对动脉粥样硬化的发病机制和斑块破裂有重大影响。本研究旨在评估缺血性心脏病患者中细菌与人体之间关于代谢物和细菌基因的相互作用的复杂性。
方法
使用实时PCR方法在全血中检测细菌的 、 、 和 基因。采用酶联免疫吸附测定法测量LL-37蛋白的浓度。对血浆中的ARA进行分析。
结果
在31%的研究患者中检测到细菌 ，在20%的患者中检测到 基因和 基因。 基因在65岁以下患者中的检测频率高于65岁及以上患者（ = 0.018），在2型糖尿病患者中的检测频率也更高（ = 0.048）。如果患者有 和生物膜特异性基因，则人抗菌肽LL-37的浓度和12S-HETE的浓度较高。
结论
本研究结果加深了对 细菌可能参与动脉粥样硬化和IHD发病机制的理解。似乎检测到了浓度较高的细菌DNA和宿主代谢物。
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