Patel Nina, Curtis Jeremy C, Plotkin Balbina J
Department of Microbiology and Immunology, College of Osteopathic Medicine, Midwestern University, Downers Grove, IL 60515, USA.
Feinberg School of Medicine, Northwestern University, Chicago, IL 60625, USA.
Antibiotics (Basel). 2021 Nov 5;10(11):1349. doi: 10.3390/antibiotics10111349.
plays an important role in biofilm formation across a wide array of disease and ecological settings. Insulin can function as an adjuvant in the regulation of biofilm levels. The modulation of insulin-regulated biofilm formation by environmental conditions has not been previously described. In the present study, the effects that various environmental growth conditions and nutrients have on insulin-modulated levels of biofilm production were measured. Micropipette tips were incubated with ATCC 25922™ in a Mueller Hinton broth (MH), or a yeast nitrogen base with 1% peptone (YNBP), which was supplemented with glucose, lactose, galactose and/or insulin (Humulin-R). The incubation conditions included a shaking or static culture, at 23 °C or 37 °C. After incubation, the biofilm production was calculated per CFU. At 23 °C, the presence of insulin increased biofilm formation. The amount of biofilm formation was highest in glucose > galactose >> lactose, while the biofilm levels decreased in shaking cultures, except for galactose (3-fold increase; 0.1% galactose and 20 μU insulin). At 37 °C, regardless of condition, there was more biofilm formation/CFU under static conditions in YNBP than in MH, except for the MH containing galactose. biofilm formation is influenced by aeration, temperature, and insulin concentration in combination with the available sugars.
在广泛的疾病和生态环境中，其在生物膜形成过程中发挥着重要作用。胰岛素可作为调节生物膜水平的佐剂。此前尚未描述环境条件对胰岛素调节生物膜形成的影响。在本研究中，测定了各种环境生长条件和营养物质对胰岛素调节的生物膜产生水平的影响。微量移液器吸头与ATCC 25922™在 Mueller Hinton肉汤（MH）或含1%蛋白胨的酵母氮碱（YNBP）中孵育，其中添加了葡萄糖、乳糖、半乳糖和/或胰岛素（优泌林-R）。孵育条件包括在23℃或37℃下振荡培养或静置培养。孵育后，计算每CFU的生物膜产生量。在23℃时，胰岛素的存在增加了生物膜的形成。生物膜形成量在葡萄糖>半乳糖>>乳糖时最高，而在振荡培养中生物膜水平降低，但半乳糖除外（增加3倍；0.1%半乳糖和20 μU胰岛素）。在37℃时，无论条件如何，在YNBP中静置条件下每CFU的生物膜形成量比在MH中更多，但含半乳糖的MH除外。生物膜形成受通气、温度、胰岛素浓度以及可用糖类的综合影响。
