Carvalho Bruna Fernandes, Gomez Gabriela Vilas Bôas, Carron Juliana, Macedo Ligia Traldi, Gonçalves Gisele Melo, Vazquez Vinicius de Lima, Serrano Sergio Vicente, Lourenço Gustavo Jacob, Lima Carmen Silvia Passos
Laboratory of Cancer Genetics, School of Medical Sciences, University of Campinas, Campinas 13083-888, SP, Brazil.
Department of Anesthesiology, Oncology, and Radiology, School of Medical Sciences, University of Campinas, Campinas 13083-888, SP, Brazil.
Int J Mol Sci. 2024 Mar 1;25(5):2868. doi: 10.3390/ijms25052868.
Regulatory T lymphocytes play a critical role in immune regulation and are involved in the aberrant cell elimination by facilitating tumor necrosis factor connection to the TNFR2 receptor, encoded by the polymorphic gene. We aimed to examine the effects of single nucleotide variants c.587T>G, c.*188A>G, c.*215C>T, and c.*922C>T on the clinicopathological characteristics and survival of cutaneous melanoma (CM) patients. Patients were genotyped using RT-PCR. levels were measured using qPCR. Luciferase reporter assay evaluated the interaction of miR-96 and miR-1271 with the 3'-UTR of . The c.587TT genotype was more common in patients younger than 54 years old than in older patients. Patients with c.*922CT or TT, c.587TG or GG + c.*922CT or TT genotypes, as well as those with the haplotype TATT, presented a higher risk of tumor progression and death due to the disease effects. Individuals with the c.*922TT genotype had a higher expression than those with the CC genotype. miR-1271 had less efficient binding with the 3'-UTR of the T allele when compared with the C allele of the SNV c.*922C>T. Our findings, for the first time, demonstrate that c.587T>G and c.*922C>T variants can serve as independent prognostic factors in CM patients.
调节性T淋巴细胞在免疫调节中起关键作用，并通过促进肿瘤坏死因子与多态性基因编码的TNFR2受体连接参与异常细胞清除。我们旨在研究单核苷酸变体c.587T>G、c.*188A>G、c.*215C>T和c.*922C>T对皮肤黑色素瘤（CM）患者临床病理特征和生存的影响。使用RT-PCR对患者进行基因分型。使用qPCR测量水平。荧光素酶报告基因检测评估miR-96和miR-1271与的3'-UTR的相互作用。c.587TT基因型在54岁以下患者中比在老年患者中更常见。携带c.*922CT或TT、c.587TG或GG + c.*922CT或TT基因型的患者，以及携带单倍型TATT的患者，由于疾病影响，肿瘤进展和死亡风险更高。携带c.*922TT基因型的个体比携带CC基因型的个体具有更高的表达。与单核苷酸变体c.*922C>T的C等位基因相比，miR-1271与T等位基因的3'-UTR结合效率更低。我们的研究结果首次表明，c.587T>G和c.*922C>T变体可作为CM患者的独立预后因素。
