Son Lina, Kost Vladimir, Maiorov Valery, Sukhov Dmitry, Arkhangelskaya Polina, Ivanov Igor, Kudryavtsev Denis, Siniavin Andrei, Utkin Yuri, Kasheverov Igor
Department of Molecular Bases of Neuroimmune Signaling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Ivanovsky Institute of Virology, N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia.
Molecules. 2024 Feb 21;29(5):943. doi: 10.3390/molecules29050943.
(LEXSY) system is an inexpensive and effective expression approach for various research and medical purposes. The stated advantages of this system are the possibility of obtaining the soluble product in the cytoplasm, a high probability of correct protein folding with a full range of post-translational modifications (including uniform glycosylation), and the possibility of expressing multi-subunit proteins. In this paper, a LEXSY expression system has been employed for obtaining the receptor binding domain (RBD) of the spike-protein of the SARS-CoV-2 virus and the homopentameric acetylcholine-binding protein (AChBP) from . RBD is actively used to obtain antibodies against the virus and in various scientific studies on the molecular mechanisms of the interaction of the virus with host cell targets. AChBP represents an excellent structural model of the ligand-binding extracellular domain of all subtypes of nicotinic acetylcholine receptors (nAChRs). Both products were obtained in a soluble glycosylated form, and their structural and functional characteristics were compared with those previously described.
（LEXSY）系统是一种用于各种研究和医学目的的经济有效的表达方法。该系统的既定优势在于有可能在细胞质中获得可溶性产物，具有在一系列完整的翻译后修饰（包括均匀糖基化）下正确蛋白质折叠的高概率，以及表达多亚基蛋白质的可能性。在本文中，LEXSY表达系统已被用于从[来源未给出]获得严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒刺突蛋白的受体结合域（RBD）和同源五聚体乙酰胆碱结合蛋白（AChBP）。RBD被积极用于获得针对该病毒的抗体以及关于病毒与宿主细胞靶标相互作用分子机制的各种科学研究中。AChBP代表了烟碱型乙酰胆碱受体（nAChRs）所有亚型的配体结合细胞外结构域的优秀结构模型。两种产物均以可溶性糖基化形式获得，并将它们的结构和功能特征与先前描述的进行了比较。
