Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, China.
Institute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, China.
Molecules. 2024 Mar 3;29(5):1136. doi: 10.3390/molecules29051136.
A chemical study of Rehd. (also called Suo Luo Zi) and the in vitro anti-inflammatory effects of the obtained compounds was conducted. Retrieving results through SciFinder showed that there were four unreported compounds, aeswilosides I-IV (-), along with fourteen known isolates (-). Their structures were elucidated by extensive spectroscopic methods such as UV, IR, NMR, [α], and MS spectra, as well as acid hydrolysis. Among the known ones, compounds , , -, and - were obtained from the genus for the first time; compounds , , , and were first identified from this plant. The NMR data of and were reported first. The effects of - on the release of nitric oxide (NO) from lipopolysaccharide (LPS)-induced RAW264.7 cells were determined. The results showed that at concentrations of 10, 25, and 50 μM, the novel compounds, aeswilosides I () and IV (), along with the known ones, 1-(2-methylbutyryl)phloroglucinyl-glucopyranoside () and pisuminic acid (), displayed significant inhibitory effects on NO production in a concentration-dependent manner. It is worth mentioning that compound showed the best NO inhibitory effect with a relative NO production of 88.1%, which was close to that of the positive drug dexamethasone. The Elisa experiment suggested that compounds , , , and suppressed the release of TNF-α and IL-1β as well. In conclusion, this study enriches the spectra of compounds with potential anti-inflammatory effects in and provides new references for the discovery of anti-inflammatory lead compounds, but further mechanistic research is still needed.
对 Rehd.（又称娑罗子）进行了化学研究，并对获得的化合物进行了体外抗炎作用研究。通过 SciFinder 检索结果表明，有四个未报道的化合物，即 aeswilosides I-IV(-)，以及十四个已知的分离物(-)。通过广泛的光谱方法，如 UV、IR、NMR、[α]和 MS 光谱以及酸水解，阐明了它们的结构。在已知的化合物中，化合物、、、和-首次从该属中获得；化合物、、、和首次从该植物中鉴定得到。首次报道了化合物和的 NMR 数据。测定了-对脂多糖（LPS）诱导的 RAW264.7 细胞中一氧化氮（NO）释放的影响。结果表明，在 10、25 和 50 μM 浓度下，新型化合物 aeswilosides I()和 IV()以及已知化合物 1-(2-甲基丁酰基)苯并呋喃葡萄糖苷()和 pisuminic 酸()，以浓度依赖的方式对 NO 的产生具有显著的抑制作用。值得注意的是，化合物显示出最好的 NO 抑制作用，相对 NO 产生率为 88.1%，接近阳性药物地塞米松。Elisa 实验表明，化合物、、、也抑制了 TNF-α和 IL-1β的释放。总之，本研究丰富了具有潜在抗炎作用的化合物在 Rehd.中的谱，为发现抗炎先导化合物提供了新的参考，但仍需要进一步的机制研究。
