Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617, Tianjin, China.
Institute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617, Tianjin, China.
Phytochemistry. 2022 Apr;196:113076. doi: 10.1016/j.phytochem.2021.113076. Epub 2022 Jan 8.
As one of raw materials, the rhizome of Imperata cylindrica var. major (Nees) C.E. Hubb. is used in kinds of preparations curing inflammation related diseases, while its effective substances are not yet clear. In this paper, its chemical constituents and their anti-inflammatory activities were investigated. As results, ten compounds, named as imperphenoside A (1), imperphenols B (2) and C (3), imperphenosides D-F (4-6), and imperlignanosides A-D (7-10), along with previously reported thirty-seven known ones (11-47) were obtained from it. Their structures were ascertained basing on the extensive spectroscopic methods and electronic circular dichroism data analysis. Meanwhile, compounds 4, 11, 12, 24, 27, 31, 32, 37, 43, 45, and 47 exhibited nitric oxide inhibitory effects in concentration dependent at 3, 10, and 30 μM on lipopolysaccharides induced RAW 264.7 cells. Moreover, the western blot analysis indicated that compounds 4, 11, 43, and 47 could restrain the phosphorylation of nuclear factor kappa-B kinase to down-regulate the protein expression of inflammatory cytokines such as inducible nitric oxide synthase, interleukin-6 and tumor necrosis factor-α. In conclusion, they might play the anti-inflammatory effects through regulating NF-κB signaling pathway.
作为一种原料，白茅根茎（Imperata cylindrica var. major（Nees）C.E. Hubb.）被用于治疗各种炎症相关疾病的制剂中，但其有效物质尚不清楚。本文对其化学成分及其抗炎活性进行了研究。结果，从白茅根茎中分离得到了 10 个化合物，命名为 imperphenoside A（1）、imperphenols B（2）和 C（3）、imperphenosides D-F（4-6）和 imperlignanosides A-D（7-10），以及之前报道的 37 个已知化合物（11-47）。它们的结构是基于广泛的光谱方法和电子圆二色谱数据分析确定的。同时，化合物 4、11、12、24、27、31、32、37、43、45 和 47 在浓度为 3、10 和 30 μM 时，对脂多糖诱导的 RAW 264.7 细胞具有浓度依赖性的一氧化氮抑制作用。此外，Western blot 分析表明，化合物 4、11、43 和 47 可以抑制核因子 kappa-B 激酶的磷酸化，从而下调诱导型一氧化氮合酶、白细胞介素-6 和肿瘤坏死因子-α 等炎症细胞因子的蛋白表达。综上所述，它们可能通过调节 NF-κB 信号通路发挥抗炎作用。
