Swiontek Brzezinska Maria, Shinde Ambika H, Kaczmarek-Szczepańska Beata, Jankiewicz Urszula, Urbaniak Joanna, Boczkowski Sławomir, Zasada Lidia, Ciesielska Magdalena, Dembińska Katarzyna, Pałubicka Krystyna, Michalska-Sionkowska Marta
Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Toruń, Poland.
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Toruń, Poland.
Polymers (Basel). 2024 Feb 20;16(5):574. doi: 10.3390/polym16050574.
Currently, natural polymer materials with bactericidal properties are extremely popular. Unfortunately, although the biopolymer material itself is biodegradable, its enrichment with bactericidal compounds may affect the efficiency of biodegradation by natural soil microflora. Therefore, the primary objective of this study was to evaluate the utility of fungi belonging to the genus in facilitating the degradation of chitosan film modified with cinnamic acid and ellagic acid in the soil environment. Only two strains (T.07 and T.14) used chitosan films as a source of carbon and nitrogen. However, their respiratory activity decreased with the addition of tested phenolic acids, especially cinnamic acid. Addition of isolates to the soil increased oxygen consumption during the biodegradation process compared with native microorganisms, especially after application of the T.07 and T.14 consortium. Isolates T.07 and T.14 showed high lipolytic (55.78 U/h and 62.21 U/h) and chitinase (43.03 U/h and 41.27 U/h) activities. Chitinase activity after incorporation of the materials into the soil was higher for samples enriched with T.07, T.14 and the consortium. The isolates were classified as sp. and Considering the outcomes derived from our findings, it is our contention that the application of isolates holds promise for expediting the degradation process of chitosan materials containing bactericidal compounds.
目前，具有杀菌特性的天然高分子材料极其受欢迎。不幸的是，尽管生物高分子材料本身是可生物降解的，但其富含的杀菌化合物可能会影响天然土壤微生物群的生物降解效率。因此，本研究的主要目的是评估属于该属的真菌在促进肉桂酸和鞣花酸改性的壳聚糖膜在土壤环境中的降解方面的效用。只有两株菌株（T.07和T.14）将壳聚糖膜用作碳源和氮源。然而，它们的呼吸活性随着测试酚酸尤其是肉桂酸的添加而降低。与天然微生物相比，向土壤中添加分离菌株增加了生物降解过程中的氧气消耗，特别是在应用T.07和T.14联合体之后。分离菌株T.07和T.14表现出较高的脂肪酶活性（分别为55.78 U/h和62.21 U/h）和几丁质酶活性（分别为43.03 U/h和41.27 U/h）。对于富含T.07、T.14和联合体的样品，将材料掺入土壤后几丁质酶活性更高。这些分离菌株被归类为 种和 考虑到我们的研究结果，我们认为应用这些分离菌株有望加速含有杀菌化合物的壳聚糖材料的降解过程。
