Hadibarata Tony, Tachibana Sanro, Itoh Kazutaka
Department of Applied Bioscience, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Pak J Biol Sci. 2007 Jun 1;10(11):1804-10. doi: 10.3923/pjbs.2007.1804.1810.
The degradation of n-eicosane by Trichoderma sp. S019, a fungus collected from soil with extensive degradative ability on an agar medium containing n-eicosane, was demonstrated in liquid medium and in soil. Maximal degradation (77%) was obtained when Trichoderma sp. S019 was incubated for 30 days after the addition of 0.1 mM of n-eicosane to the liquid medium while the highest rate of degradation (40%) was obtained in soil with the addition of 1.5% Trichoderma sp. S019. Furthermore, the degradation of n-eicosane was affected by the addition of a carbon source, the addition of a nitrogen source and agitation. n-Eicosane was indeed degraded by Trichoderma sp. S019 because nonadecanoic acid, n-octadecane, hexadecanoic acid, oleic acid and stearic acid, considered to be the intermediates in the biodegradation of n-eicosane, were detected among the reaction products.
从土壤中分离得到的木霉菌株Trichoderma sp. S019对正二十烷具有广泛的降解能力，在含正二十烷的琼脂培养基上生长良好。该菌株在液体培养基和土壤中对正二十烷的降解能力得到了验证。在液体培养基中添加0.1 mM正二十烷后，将Trichoderma sp. S019培养30天，可实现最大降解率（77%）；而在土壤中添加1.5%的Trichoderma sp. S019时，降解率最高可达40%。此外，碳源、氮源的添加以及振荡均会影响正二十烷的降解。Trichoderma sp. S019确实能够降解正二十烷，因为在反应产物中检测到了被认为是正二十烷生物降解中间产物的十九烷酸、正十八烷、十六烷酸、油酸和硬脂酸。
