Štěpánková Kateřina, Ozaltin Kadir, Sáha Petr, Vargun Elif, Domincová-Bergerová Eva, Vesel Alenka, Mozetič Miran, Lehocký Marian
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlin, Czech Republic.
Department of Chemistry, Mugla Sitki Kocman University, Kotekli, 48000 Mugla, Turkey.
Polymers (Basel). 2024 Mar 6;16(5):720. doi: 10.3390/polym16050720.
This study involved the creation of highly porous PLA scaffolds through the porogen/leaching method, utilizing polyethylene glycol as a porogen with a 75% mass ratio. The outcome achieved a highly interconnected porous structure with a thickness of 25 μm. To activate the scaffold's surface and improve its hydrophilicity, radiofrequency (RF) air plasma treatment was employed. Subsequently, furcellaran subjected to sulfation or carboxymethylation was deposited onto the RF plasma treated surfaces with the intention of improving bioactivity. Surface roughness and water wettability experienced enhancement following the surface modification. The incorporation of sulfate/carboxymethyl group (DS = 0.8; 0.3, respectively) is confirmed by elemental analysis and FT-IR. Successful functionalization of PLA scaffolds was validated by SEM and XPS analysis, showing changes in topography and increases in characteristic elements (N, S, Na) for sulfated (SF) and carboxymethylated (CMF). Cytocompatibility was evaluated by using mouse embryonic fibroblast cells (NIH/3T3).
本研究通过致孔剂/浸出法制备了高度多孔的聚乳酸（PLA）支架，使用质量比为75%的聚乙二醇作为致孔剂。结果得到了具有高度相互连通的多孔结构、厚度为25μm的支架。为了活化支架表面并提高其亲水性，采用了射频（RF）空气等离子体处理。随后，将经过硫酸化或羧甲基化处理的角叉菜聚糖沉积在经过RF等离子体处理的表面上，以提高生物活性。表面改性后，表面粗糙度和水润湿性得到了改善。通过元素分析和傅里叶变换红外光谱（FT-IR）证实了硫酸根/羧甲基基团（取代度分别为0.8和0.3）的引入。通过扫描电子显微镜（SEM）和X射线光电子能谱（XPS）分析验证了PLA支架的成功功能化，显示了硫酸化（SF）和羧甲基化（CMF）支架在形貌上的变化以及特征元素（N、S、Na）的增加。使用小鼠胚胎成纤维细胞（NIH/3T3）评估了细胞相容性。
