Chi Wenwei, Hu Lili, Li Zhiwen, Lin Borong, Zhuo Kan, Liao Jinling
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, China.
Plants (Basel). 2024 Mar 4;13(5):730. doi: 10.3390/plants13050730.
is one of the most widespread and economically important sedentary endoparasites. In this study, a comparative transcriptome analysis of between pre-parasitic second-stage juveniles (Pre-J2) and parasitic juveniles (Par-J3/J4) was conducted. A total of 48,698 unigenes were obtained, of which 18,826 genes showed significant differences in expression ( < 0.05). In the differentially expressed genes (DEGs) from transcriptome data at Par-J3/J4 and Pre-J2, a large number of unigenes were annotated to the C-type lectin (CTL, ), the cathepsin L-like protease (), the venom allergen-like protein (), and the cellulase (endo-β-1,4-glucanase). Among seven types of lectins found in the DEGs, there were 10 CTLs. The regulatory roles of , and in plant immune responses involved in the parasitism of were investigated. The results revealed that could suppress programmed cell death (PCD) triggered by Gpa2/RBP-1 and inhibit the flg22-stimulated ROS burst. In situ hybridization and developmental expression analyses showed that was specifically expressed in the subventral gland of , and its expression was up-regulated at Pre-J2 of the nematode. In addition, silencing of substantially increased the plant resistance to . Moreover, yeast co-transformation and bimolecular fluorescence complementation assay showed that specifically interacted with the catalase, . It was demonstrated that could suppress the innate immunity of plants through the peroxide system, thereby promoting parasitism.
是分布最广泛且在经济上最重要的定居性内寄生生物之一。在本研究中，对寄生前的第二阶段幼虫（Pre-J2）和寄生性幼虫（Par-J3/J4）进行了比较转录组分析。共获得48,698个单基因，其中18,826个基因表达存在显著差异（<0.05）。在Par-J3/J4和Pre-J2转录组数据的差异表达基因（DEG）中，大量单基因被注释为C型凝集素（CTL）、组织蛋白酶L样蛋白酶（）、毒液过敏原样蛋白（）和纤维素酶（内切-β-1,4-葡聚糖酶）。在DEG中发现的七种凝集素类型中，有10个CTL。研究了、和在参与寄生的植物免疫反应中的调节作用。结果表明，可抑制由Gpa2/RBP-1触发的程序性细胞死亡（PCD），并抑制flg22刺激的活性氧爆发。原位杂交和发育表达分析表明，在的腹侧下腺中特异性表达，且其表达在该线虫的Pre-J2阶段上调。此外，沉默显著提高了植物对的抗性。而且，酵母共转化和双分子荧光互补分析表明，与过氧化氢酶特异性相互作用。证明可通过过氧化物系统抑制植物的先天免疫，从而促进寄生。
