The State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu, China.
School of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu, China.
Cell Biochem Funct. 2024 Mar;42(2):e3975. doi: 10.1002/cbf.3975.
Different organic compounds can have varying degrees of impact on the activity of Lactobacillus paracasei. The study focused on the impact and action mechanism of different organic selenium products on the bioactivity of two strains of L. paracasei. The growth, antioxidant activity, extracellular polysaccharide secretion, quorum sensing (QS), and biofilm formation of the strains before and after the addition of organic selenium crude products and three organic selenium standard were evaluated. The results showed that the addition of crude organic selenium promoted the various activities of the strain. l-selenocysteine had the strongest regulatory effect, with maximum GIM1.80 biofilm formation when it reached a critical concentration of 0.4 μg/mL; l-selenomethionine resulted in the highest activity of the signal molecule Auto inducer-2 of GDMCC1.155, when it reached a critical concentration of 0.4 μg/mL. The results of scanning electron microscopy demonstrated that the addition of organic selenium effectively improved the morphological structure of the two bacterial cells. Molecular docking revealed that the mechanism by which organic selenium regulates QS in Lactobacillus was achieved by binding two crucial receptor proteins (histidine protein kinase HKP and periplasmic binding protein LuxP) from specific sites. Furthermore, organic selenium products have a beneficial regulatory effect on the biological activity of L. paracasei. Overall, these findings provide a new alternative (organic selenium) for regulating the viability and beneficial activity of L. paracasei.
不同的有机化合物对副干酪乳杆菌的活性有不同程度的影响。本研究重点研究了不同有机硒产品对两株副干酪乳杆菌生物活性的影响和作用机制。评估了添加有机硒粗产品和三种有机硒标准品前后菌株的生长、抗氧化活性、胞外多糖分泌、群体感应（QS）和生物膜形成情况。结果表明，添加粗有机硒促进了菌株的各种活性。l-硒代半胱氨酸具有最强的调节作用，当达到 0.4μg/ml 的临界浓度时，GIM1.80 生物膜形成达到最大值；l-硒代蛋氨酸使 GDMCC1.155 的信号分子 Auto inducer-2 的活性最高，当达到 0.4μg/ml 的临界浓度时。扫描电子显微镜的结果表明，有机硒的添加有效地改善了两种细菌细胞的形态结构。分子对接表明，有机硒调节乳杆菌 QS 的机制是通过与两个特定位点的关键受体蛋白（组氨酸蛋白激酶 HKP 和周质结合蛋白 LuxP）结合来实现的。此外，有机硒产品对副干酪乳杆菌的生物活性具有有益的调节作用。总的来说，这些发现为调节副干酪乳杆菌的生存能力和有益活性提供了一种新的选择（有机硒）。
