Department of Dermatology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Department of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
Drug Des Devel Ther. 2024 Mar 5;18:719-729. doi: 10.2147/DDDT.S423974. eCollection 2024.
Capsaicin is the main compound found in chili pepper and has complex pharmacologic effects. This study aimed to elucidate the mechanism of the effect of capsaicin on physiological processes by analyzing changes in metabolites and metabolic pathways.
Female C57BL/6 mice were divided into two groups(n = 10/group) and fed with capsaicin-soybean oil solution(group T) or soybean oil(group C) for 6 weeks. Ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-qTOF-MS) based metabolomics was undertaken to assess plasma and skin metabolic profile changes and identify differential metabolites through multivariate analysis.
According to the OPLS-DA score plots, the plasma and skin metabolic profiles in the group T and group C were significantly separated. In plasma, 38 significant differential metabolites were identified. KEGG pathway enrichment analysis revealed that the most significant plasma metabolic pathways included pyruvate metabolism and ABC transporters. In skin, seven significant differential metabolites were found. Four metabolic pathways with p values < 0.05 were detected, including sphingolipid metabolism, sphingolipid signaling pathway, apoptosis, and necroptosis.
These findings will provide metabolomic insights to assess the physiological functions of capsaicin and contribute to a better understanding of the potential effects of a capsaicin-rich diet on health.
辣椒素是辣椒中的主要化合物，具有复杂的药理作用。本研究旨在通过分析代谢物和代谢途径的变化，阐明辣椒素对生理过程的影响机制。
将雌性 C57BL/6 小鼠分为两组（每组 n = 10），分别用辣椒素-大豆油溶液（T 组）或大豆油（C 组）喂养 6 周。采用超高效液相色谱/四极杆飞行时间质谱（UHPLC-qTOF-MS）代谢组学方法评估血浆和皮肤代谢谱的变化，并通过多元分析鉴定差异代谢物。
根据 OPLS-DA 得分图，T 组和 C 组的血浆和皮肤代谢谱明显分离。在血浆中，鉴定出 38 种显著差异代谢物。KEGG 通路富集分析显示，最显著的血浆代谢途径包括丙酮酸代谢和 ABC 转运体。在皮肤中，发现了 7 种显著差异代谢物。检测到 4 条代谢途径的 p 值<0.05，包括鞘脂代谢、鞘脂信号通路、细胞凋亡和坏死性凋亡。
这些发现将为评估辣椒素的生理功能提供代谢组学见解，并有助于更好地了解富含辣椒素的饮食对健康的潜在影响。
