Jaiswal Anugya, Khan Aquib, Yogi Akanksha, Singh Sweta, Pal Arun Kumar, Soni Ramendra, Tripathi Pooja, Lal Jonathan A, Tripathi Vijay
Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India.
Center of Computational Biology and Bioinformatics, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India.
3 Biotech. 2024 Apr;14(4):107. doi: 10.1007/s13205-024-03950-7. Epub 2024 Mar 10.
Antibiotics in animal farms play a significant role in the proliferation and spread of antibiotic-resistant genes (ARGs) and antibiotic-resistant bacteria (ARB). The dissemination of antibiotic resistance from animal facilities to the nearby environment has become an emerging concern. The present study was focused on the isolation and molecular identification of () isolates from broiler chicken meat and further access their antibiotic-resistant profile against different antibiotics. Broiler chicken meat samples were collected from 44 retail poultry slaughter shops in Prayagraj district, Uttar Pradesh, India. Standard bacteriological protocols were followed to first isolate the , and molecular characterization was performed with genus-specific PCR. Phenotypic and genotypic antibiotic-resistant profiles of all confirmed 154 isolates were screened against 09 antibiotics using the disc diffusion and PCR-based method for selected resistance genes. In antibiotic sensitivity testing, the isolates have shown maximum resistance potential against tetracycline (78%), ciprofloxacin (57.8%), trimethoprim (54.00%) and erythromycin (49.35%). bacterial isolates have shown relative resistant to amoxicillin-clavulanic acid (43.00%) and against ampicillin (44.15%). Notably, 64.28% bacteria were found to be multidrug resistant. The results of PCR assays exposed that tetA and blaTEM genes were the most abundant genes harboured by 83 (84.0%) and 82 (82.0%) out of all 99 targeted isolates, followed by 48.0% for AmpC (CITM) gene and cmlA (23.00%) for chloramphenicol resistance. It is notable that most of the isolates collected from chicken meat samples were multidrug resistant (> 3 antibiotics), with more than 80% of them carrying tetracycline (tetA) and beta-lactam gene (blaTEM). This study highlights the high risk associated with poultry products due to MDR- and promote the limited use of antibiotics in poultry farms.
The online version contains supplementary material available at 10.1007/s13205-024-03950-7.
养殖场中的抗生素在抗生素抗性基因（ARGs）和抗生素抗性细菌（ARB）的增殖与传播中起着重要作用。抗生素抗性从动物养殖设施传播到附近环境已成为一个新出现的问题。本研究聚焦于从肉鸡中分离和分子鉴定（）分离株，并进一步评估它们对不同抗生素的抗性谱。从印度北方邦普拉亚格拉杰区的44家零售家禽屠宰店采集了肉鸡样本。遵循标准细菌学方案首先分离（），并通过属特异性PCR进行分子表征。使用纸片扩散法和基于PCR的选定抗性基因方法，针对9种抗生素筛选了所有154株已确认的（）分离株的表型和基因型抗生素抗性谱。在抗生素敏感性测试中，分离株对四环素（78%）、环丙沙星（57.8%）、甲氧苄啶（54.00%）和红霉素（49.35%）表现出最大抗性潜力。（）细菌分离株对阿莫西林 - 克拉维酸（43.00%）和氨苄西林（44.15%）表现出相对抗性。值得注意的是，发现64.28%的（）细菌具有多重耐药性。PCR检测结果表明，tetA和blaTEM基因是所有99株目标（）分离株中83株（84.0%）和82株（82.0%）携带最多的基因，其次是AmpC（CITM）基因的携带率为48.0%，氯霉素抗性的cmlA基因携带率为23.00%。值得注意的是，从鸡肉样本中收集的大多数分离株具有多重耐药性（>3种抗生素），其中超过80%携带四环素（tetA）和β - 内酰胺基因（blaTEM）。本研究强调了由于多重耐药性与家禽产品相关的高风险，并提倡在家禽养殖场有限使用抗生素。
在线版本包含可在10.1007/s13205 - 024 - 03950 - 7获取的补充材料。
