Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.
Section of Hematology and Medical Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Methods Mol Biol. 2024;2783:177-193. doi: 10.1007/978-1-0716-3762-3_12.
With the increase in decellularization of different tissue sources, an understanding of the viscoelastic properties of these soft materials is important for determining practical applications. The purpose of this chapter is to better define a series of experiments to profile important rheological properties for adipose-based hydrogels. While there are numerous mechanical characterizations that are done experimentally, the protocol outlined in this chapter provides a step-wise approach to determine the gelation characteristics and native hydrogel network properties. A more complete understanding of adipose-derived hydrogel mechanical properties would provide vital information for downstream applicability in fields such as disease modeling or soft tissue regeneration.
随着不同组织来源的脱细胞化程度的增加，了解这些软材料的黏弹性特性对于确定实际应用非常重要。本章的目的是更好地定义一系列实验，以描绘基于脂肪的水凝胶的重要流变特性。虽然有许多机械特性是通过实验来进行的，但本章中概述的方案提供了一种逐步的方法来确定凝胶化特性和天然水凝胶网络特性。对脂肪衍生水凝胶机械性能的更全面了解将为疾病建模或软组织再生等领域的下游应用提供重要信息。
