Division of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Feigin Center at Texas Children's Hospital, 1102 Bates Ave, C.450.05, Houston, Texas 77030, United States of America.
Department of Biological Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United States of America.
ACS Appl Mater Interfaces. 2023 Apr 19;15(15):18639-18652. doi: 10.1021/acsami.2c22982. Epub 2023 Apr 6.
The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignin to confer antioxidation to the extracellular microenvironments of wounds and to deliver oxygen from the dissociation of calcium peroxide for enhanced vascularization and healing responses without eliciting inflammatory responses. Elemental analysis showed 17 times higher quantity of calcium in the oxygen-releasing nanoparticles. Lignin composites including the oxygen-generating nanoparticles released around 700 ppm oxygen per day at least for 7 days. By modulating the concentration of the methacrylated gelatin, we were able to maintain the injectability of lignin composite precursors and the stiffness of lignin composites suitable for wound healing after photo-cross-linking. formation of lignin composites with the oxygen-releasing nanoparticles enhanced the rate of tissue granulation, the formation of blood vessels, and the infiltration of α-smooth muscle actin fibroblasts into the wounds over 7 days. At 28 days after surgery, the lignin composite with oxygen-generating nanoparticles remodeled the collagen architecture, resembling the basket-weave pattern of unwounded collagen with minimal scar formation. Thus, our study shows the potential of functionalized lignin for wound-healing applications requiring balanced antioxidation and controlled release of oxygen for enhanced tissue granulation, vascularization, and maturation of collagen.
自组织工程学诞生以来，人们一直在研究工程生物材料在伤口愈合中的应用。在这里，我们尝试将功能化木质素应用于赋予伤口细胞外微环境抗氧化能力，并通过过氧化钙的解离来输送氧气，以促进血管生成和愈合反应，而不会引发炎症反应。元素分析显示，释氧纳米颗粒中的钙含量高出 17 倍。木质素复合材料包括产氧纳米颗粒，每天至少释放 700ppm 的氧气，持续至少 7 天。通过调节甲基丙烯酰化明胶的浓度，我们能够保持木质素复合材料前体的可注射性和光交联后适合伤口愈合的木质素复合材料的刚度。形成木质素复合材料与释氧纳米颗粒增强了组织肉芽形成的速度，血管的形成，以及α-平滑肌肌动蛋白成纤维细胞在 7 天内渗透到伤口中。在手术后 28 天，具有供氧纳米颗粒的木质素复合材料重塑了胶原结构，类似于未受伤胶原的篮状图案，形成的疤痕最小。因此，我们的研究表明，功能化木质素具有用于伤口愈合的应用潜力，需要平衡抗氧化和控制氧气释放，以增强组织肉芽形成、血管生成和胶原成熟。
