Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt.
Applied and Environmental Microbiology Center, South Valley University, Qena, Egypt.
Sci Rep. 2024 Mar 13;14(1):6076. doi: 10.1038/s41598-024-53849-9.
When onions are improperly stored, a post-harvest disease known as black mold of onion bulbs can result in considerable economic losses. Aspergillus section Nigri, one of many species, has been implicated in the development of black mold. In the present study, rot onion bulbs were collected from markets in Qena, Egypt. Thirteen Aspergillus section Nigri isolates were obtained and identified by morphological and molecular characterization. The ochratoxins potential of isolated A. section Nigri was tested, and three isolates were producers at the range of 1.5-15 ppm. For the presence of pks gene, no amplification product was detected. Using the fungal growth inhibition test, the isolates of A. niger were inhibited by eco-friendly materials Cement and Zeolite. Cement exhibited maximum percentage growth inhibition against the tested isolates at 74.7-86.7%. The pathogenicity activity of the A. niger isolates was tested by inoculation of healthy onion bulbs, other onion bulbs covered with Cement and Zeolite before inoculation by A. niger was used. The two treatments significantly reduced bulbs rot disease of onion than untreated bulbs. Seven and nine isolates showed 0% rot on covered bulbs by Cement and Zeolite, respectively as compared with inoculated onions, which exhibited rot ranging from 55 to 80%. Using eco-friendly materials with efficiency against post-harvest bulbs rot of onion was evaluated in this study.
当洋葱储存不当时，一种称为洋葱鳞茎黑霉病的采后病害会导致相当大的经济损失。黑曲霉属的许多种中的一种，被认为与黑霉病的发生有关。在本研究中，从埃及盖纳的市场上收集了腐烂的洋葱鳞茎。获得了 13 个黑曲霉属段的分离物，并通过形态和分子特征进行了鉴定。测试了分离的黑曲霉属段的赭曲霉毒素潜力，发现有三个分离物的产生范围在 1.5-15ppm。对于 pks 基因的存在，没有检测到扩增产物。使用真菌生长抑制试验，黑曲霉属的分离物被环保材料水泥和沸石抑制。水泥对测试分离物的抑制率最大，为 74.7-86.7%。通过接种健康的洋葱鳞茎，测试了黑曲霉属分离物的致病性活性，在接种黑曲霉属之前，洋葱鳞茎先覆盖水泥和沸石。与未处理的鳞茎相比，这两种处理方法显著降低了洋葱鳞茎腐烂病的发病率。覆盖有水泥和沸石的鳞茎上有 7 个和 9 个分离物的腐烂率分别为 0%，而未覆盖的鳞茎上的腐烂率为 55-80%。本研究评估了使用对洋葱采后鳞茎腐烂有防治效果的环保材料。
