Thompson Rachelle N, Pearson Erin, McDonough Sean P, Iannitti Helenrose, Van de Walle Gerlinde R, Banse Heidi, Perkins Gillian A, Tomlinson Joy E
Department of Clinical Sciences, Cornell University, Ithaca, NY.
Department of Population Medicine, Cornell University, Ithaca, NY.
Am J Vet Res. 2024 Mar 18;85(6). doi: 10.2460/ajvr.23.12.0282. Print 2024 Jun 1.
To investigate the role of equine herpesvirus-2 (EHV-2) and equine herpesvirus-5 (EHV-5) in equine glandular gastric disease (EGGD) by visualizing and quantifying these gamma herpesviruses in EGGD-affected and normal glandular gastric mucosa of horses. A secondary objective was to describe the histopathological abnormalities in the equine gastric glandular mucosa in horses with EGGD.
29 horses (n = 21 postmortem and 8 gastroscopy) categorized as normal (11), EGGD (12), or both EGGD and equine squamous gastric disease (6).
Glandular gastric mucosal samples were collected from horses by gastroscopy or postmortem. Histopathology and in situ hybridization targeting EHV-2 and EHV-5 were performed on grossly normal and abnormal glandular gastric mucosa. The number of in situ hybridization-positive cells per millimeter squared of tissue was calculated. Evaluators were blinded to groups.
Glandular gastric tissues from horses without EGGD had higher viral loads in the mucosa than normal or abnormal tissues from EGGD horses. There was no difference in viral loads for EHV-2 or EHV-5 between grossly or endoscopically normal to abnormal gastric tissues within horses with EGGD. Lymphocytic plasmacytic gastritis was the most common histopathological abnormality, with only 3 horses having mucosal disruption (glandular ulcer or erosion).
Equine gamma herpesviruses are unlikely to play a role in the pathophysiology of EGGD. EGGD is frequently inflammatory with occasional mucosal disruption (ulcer or erosion).
通过对受马腺胃炎（EGGD）影响的和正常的马腺胃黏膜中的这些γ疱疹病毒进行可视化和定量分析，研究马疱疹病毒2型（EHV - 2）和马疱疹病毒5型（EHV - 5）在马腺胃炎（EGGD）中的作用。第二个目的是描述患有EGGD的马的胃腺黏膜中的组织病理学异常。
29匹马（n = 21匹为死后样本，8匹为胃镜检查样本），分为正常组（11匹）、EGGD组（12匹）或EGGD和马鳞状胃炎组（6匹）。
通过胃镜检查或死后解剖从马身上采集腺胃黏膜样本。对大体正常和异常的腺胃黏膜进行组织病理学检查以及针对EHV - 2和EHV - 5的原位杂交。计算每平方毫米组织中原位杂交阳性细胞的数量。评估人员对分组情况不知情。
没有EGGD的马的腺胃组织黏膜中的病毒载量高于EGGD马的正常或异常组织。在患有EGGD的马中，无论大体或内镜检查显示胃组织正常或异常，EHV - 2或EHV - 5的病毒载量均无差异。淋巴细胞性浆细胞性胃炎是最常见的组织病理学异常，只有3匹马有黏膜破坏（腺溃疡或糜烂）。
马γ疱疹病毒不太可能在EGGD的病理生理学中起作用。EGGD通常是炎症性的，偶尔有黏膜破坏（溃疡或糜烂）。
