Voss Sarah J, McGuinness David H, Weir William, Sutton David G M
School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
J Equine Vet Sci. 2022 Sep;116:104002. doi: 10.1016/j.jevs.2022.104002. Epub 2022 Apr 29.
The role of the equine gastrointestinal microbiota in the pathogenesis of equine glandular gastric disease (EGGD) is poorly understood. To investigate whether the glandular gastric microbiota is altered in horses with EGGD. Prospective longitudinal study. Five Thoroughbred racehorses from one training center underwent gastroscopy as part of poor performance investigation. Samples were taken from EGGD lesions and adjacent normal mucosa using sheathed transendoscopic cytology brushes and frozen at -80°C. DNA was extracted for 16S rRNA sequencing, and sequences compared against a database to generate taxonomic classification of the microbiota. The same horses were sampled 6 months later. Normal glandular mucosal samples were characterized by a higher proportion of Proteobacteria (46.3%) than EGGD lesions (18.9%). Relative abundance of Firmicutes was lower in samples from normal mucosa (20.0%) than EGGD lesions (41.2%). Linear discriminant analysis effect size (LEfSe) confirmed a greater proportion of Firmicutes species was characteristic of samples collected from EGGD lesions due to a very high relative abundance of Sarcina (up to 92.4%) in two horses with EGGD. We were unable to comment on the stability of the glandular gastric microbiota over time. Small sample population. None of the horses examined had grossly normal gastric mucosa. The gastric microbiota appears altered in EGGD, although we are unable to demonstrate a causative effect. Sarcina was particularly increased in abundance in EGGD and may be a useful biomarker of disease. Sheathed cytology brushes were an effective method for sampling the gastric mucosa.
马胃肠道微生物群在马腺胃疾病（EGGD）发病机制中的作用尚不清楚。为了研究EGGD马匹的腺胃微生物群是否发生改变。进行前瞻性纵向研究。来自一个训练中心的五匹纯种赛马作为表现不佳调查的一部分接受了胃镜检查。使用带鞘的经内镜细胞学刷从EGGD病变和相邻正常黏膜取样，并在-80°C下冷冻。提取DNA进行16S rRNA测序，并将序列与数据库进行比较以生成微生物群的分类学分类。6个月后对同一批马进行取样。正常腺黏膜样本中变形菌门的比例（46.3%）高于EGGD病变（18.9%）。正常黏膜样本中厚壁菌门的相对丰度（20.0%）低于EGGD病变（41.2%）。线性判别分析效应大小（LEfSe）证实，由于两匹患有EGGD的马中八叠球菌的相对丰度非常高（高达92.4%），厚壁菌门物种在从EGGD病变采集的样本中占更大比例。我们无法对腺胃微生物群随时间的稳定性发表评论。样本量小。所检查的马匹均没有大体正常的胃黏膜。EGGD中胃微生物群似乎发生了改变，尽管我们无法证明其因果关系。八叠球菌在EGGD中的丰度特别增加，可能是一种有用的疾病生物标志物。带鞘细胞学刷是一种有效的胃黏膜取样方法。
