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COVID-19 患者的血浆不会改变体外人血脑屏障的电阻。

Plasma of COVID-19 Patients Does Not Alter Electrical Resistance of Human Endothelial Blood-Brain Barrier In Vitro.

机构信息

Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, LT-01102 Vilnius, Lithuania.

Faculty of Medicine, Vilnius University, LT-03101 Vilnius, Lithuania.

出版信息

Function (Oxf). 2024 Jan 9;5(2):zqae002. doi: 10.1093/function/zqae002. eCollection 2024.

DOI:10.1093/function/zqae002
PMID:38486975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10935481/
Abstract

The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 instigated the most serious global health crisis. Clinical presentation of COVID-19 frequently includes severe neurological and neuropsychiatric symptoms. However, it is presently unknown whether and to which extent pathological impairment of blood-brain barrier (BBB) contributes to the development of neuropathology during COVID-19 progression. In the present study, we used human induced pluripotent stem cells-derived brain endothelial cells (iBECs) to study the effects of blood plasma derived from COVID-19 patients on the BBB integrity in vitro. We also performed a comprehensive analysis of the cytokine and chemokine profiles in the plasma of COVID-19 patients, healthy and recovered individuals. We found significantly increased levels of interferon γ-induced protein 10 kDa, hepatocyte growth factor, and interleukin-18 in the plasma of COVID-19 patients. However, blood plasma from COVID-19 patients did not affect transendothelial electrical resistance in iBEC monolayers. Our results demonstrate that COVID-19-associated blood plasma inflammatory factors do not affect BBB paracellular pathway directly and suggest that pathological remodeling (if any) of BBB during COVID-19 may occur through indirect or yet unknown mechanisms.

摘要

由严重急性呼吸综合征冠状病毒 2 引起的 2019 年冠状病毒病（COVID-19）大流行引发了最严重的全球卫生危机。COVID-19 的临床特征经常包括严重的神经和神经精神症状。然而，目前尚不清楚病理上血脑屏障（BBB）的损伤是否以及在多大程度上促进了 COVID-19 进展过程中的神经病理学发展。在本研究中，我们使用人类诱导多能干细胞衍生的脑内皮细胞（iBECs）来研究源自 COVID-19 患者的血浆对体外 BBB 完整性的影响。我们还对 COVID-19 患者、健康人和康复者的血浆中的细胞因子和趋化因子谱进行了全面分析。我们发现 COVID-19 患者血浆中干扰素 γ诱导蛋白 10 kDa、肝细胞生长因子和白细胞介素 18 的水平显著升高。然而，COVID-19 患者的血浆并未影响 iBEC 单层细胞的跨内皮电阻。我们的结果表明，与 COVID-19 相关的血浆炎症因子不会直接影响 BBB 的旁细胞途径，并表明 COVID-19 期间 BBB 的病理性重塑（如果有）可能通过间接或未知的机制发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/a5e7bdc2f219/zqae002fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/32f4535eef7d/zqae002fig1g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/12cab3fd33b6/zqae002fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/da1770589435/zqae002fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/a5e7bdc2f219/zqae002fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/32f4535eef7d/zqae002fig1g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/12cab3fd33b6/zqae002fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/da1770589435/zqae002fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bce/10935481/a5e7bdc2f219/zqae002fig3.jpg

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本文引用的文献

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