Fiama das Neves Ortiz Sharmila, Verdan Raphael, Rocha Gustavo Miranda, Miranda Kildare, Benchimol Marlene
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Unidade de Pesquisa Urogenital, Centro Biomédico, Departamento de Anatomia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil.
J Struct Biol X. 2024 Mar 6;9:100099. doi: 10.1016/j.yjsbx.2024.100099. eCollection 2024 Jun.
is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a complex and elaborate cytoskeleton constituting the mastigont system. The mastigont system is mainly formed by several proteinaceous structures associated with basal bodies, the pelta-axostylar complex made of microtubules, and striated filaments named the costa and the parabasal filaments (PFs). Although the structural organization of trichomonad cytoskeletons has been analyzed using several techniques, observation using a new generation of scanning electron microscopes with a resolution exceeding 1 nm has allowed more detailed visualization of the three-dimensional organization of the mastigont system. In this study, we have investigated the cytoskeleton of using a diverse range of scanning probe microscopy techniques, which were complemented by electron tomography and Fast-Fourier methods. This multi-modal approach has allowed us to characterize an unknown parabasal filament and reveal the ultrastructure of other striated fibers that have not been published before. Here, we show the differences in origin, striation pattern, size, localization, and additional details of the PFs, thus improving the knowledge of the cell biology of this parasite.
是滴虫病的病原体，滴虫病是全球最常见的非病毒性性传播感染，估计每年有2.6亿新病例。它含有所有真核细胞共有的细胞器、如氢化酶体等不常见的细胞结构，以及构成鞭毛系统的复杂精细的细胞骨架。鞭毛系统主要由几种与基体相关的蛋白质结构、由微管组成的盾 - 轴杆复合体以及名为肋和副基丝（PFs）的横纹丝组成。尽管已经使用多种技术分析了滴虫细胞骨架的结构组织，但使用分辨率超过1纳米的新一代扫描电子显微镜进行观察，能够更详细地可视化鞭毛系统的三维组织。在本研究中，我们使用了多种扫描探针显微镜技术研究了的细胞骨架，并辅以电子断层扫描和快速傅里叶方法。这种多模态方法使我们能够表征一种未知的副基丝，并揭示以前未发表的其他横纹纤维的超微结构。在这里，我们展示了副基丝在起源、条纹模式、大小、定位和其他细节方面的差异，从而增进了对这种寄生虫细胞生物学的了解。
