Lee Kyung Eun, Kim Ji Hui, Jung Min Kyo, Arii Tatsuo, Ryu Jae-Sook, Han Sung Sik
School of Life Sciences and Biotechnology, Korea University, 1, 5 Anamdong, Seongbukgu, Seoul, Korea.
J Electron Microsc (Tokyo). 2009 Oct;58(5):305-13. doi: 10.1093/jmicro/dfp019. Epub 2009 Apr 22.
The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.
由于其医学意义和独特结构，有鞭毛的原生动物阴道毛滴虫受到了广泛研究。通过连续切片重建了阴道毛滴虫细胞成分复杂的三维（3D）结构，以便观察整个细胞的空间特征。利用电子断层扫描来检查细胞器的详细结构。断层重建详细显示了阴道毛滴虫的鞭毛器系统和副基丝。最后一根细丝（Pf3）靠近相邻细丝，在横截面视图中这两根细丝似乎垂直平行。在透射电子显微镜获得的二维图像中，Pf3可能无法与相邻细丝区分开来。我们对阴道毛滴虫的三维重建揭示了存在一条额外的横纹纤维，并且通过电子断层扫描进行的三维重建显示了分裂的副基丝发生扭曲。
