Mehdikhani Fariba, Atashbasteh Mostafa, Azadi Mehdi, Allameh Abdolamir
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Oral Dis. 2024 Nov;30(8):4838-4846. doi: 10.1111/odi.14933. Epub 2024 Mar 15.
Fibroblast growth factor receptor-2 (FGFR2) and miR-889-3p expression in oral squamous cell carcinoma (OSCC) tumours were compared to normal controls. We then examined the relationship between miR-889-3p, FGFR2 expression and patient clinicopathological features.
The interaction of FGFR2 and miR-889-3p was investigated using bioinformatics. Then, OSCC tumour biopsies and normal gingiva were collected and processed for expression analysis of FGFR2-specific mRNA and miR-889-3p using real-time PCR. Immunohistochemistry evaluated the expression of the FGFR2 protein.
The protein and mRNA expression levels of FGFR2 were significantly greater in tumours when contrasted with controls. Expression of miR-889-3p was significantly lower in OSCC compared to normal tissues. The FGFR2 and miR-889-3p expressions were inversely related (-0.86 and -0.73, respectively) in both cases and controls. Changes in miR-889-3p and FGFR2 expression in tumour tissues were associated with lymph node metastasis (LNM), with ~0.57 and ~3.0 folds of change in positive-LNM patients, respectively.
Decreased expression of miR-889-3p in OSCC tumours suggests that miR-889-3p functions as a tumour suppressor gene. Overexpression of FGFR2 further proves the role of miR-889-3p in the regulation of the FGFR2 pathway. This was further confirmed by showing differences in miR-889-3p expression in positive and negative LNM cases.
比较口腔鳞状细胞癌（OSCC）肿瘤组织与正常对照中纤维母细胞生长因子受体2（FGFR2）和miR - 889 - 3p的表达情况。然后，我们研究了miR - 889 - 3p、FGFR2表达与患者临床病理特征之间的关系。
利用生物信息学研究FGFR2与miR - 889 - 3p的相互作用。然后，收集OSCC肿瘤活检组织和正常牙龈组织，采用实时荧光定量PCR对FGFR2特异性mRNA和miR - 889 - 3p进行表达分析。免疫组织化学法评估FGFR2蛋白的表达。
与对照组相比，肿瘤组织中FGFR2的蛋白和mRNA表达水平显著更高。与正常组织相比，OSCC中miR - 889 - 3p的表达显著降低。在病例组和对照组中，FGFR2与miR - 889 - 3p的表达均呈负相关（分别为-0.86和-0.73）。肿瘤组织中miR - 889 - 3p和FGFR2表达的变化与淋巴结转移（LNM）相关，在LNM阳性患者中，miR - 889 - 3p和FGFR2的变化倍数分别约为0.57和3.0倍。
OSCC肿瘤中miR - 889 - 3p表达降低表明miR - 889 - 3p作为肿瘤抑制基因发挥作用。FGFR2的过表达进一步证明了miR - 889 - 3p在FGFR2通路调控中的作用。LNM阳性和阴性病例中miR - 889 - 3p表达的差异进一步证实了这一点。
