Yang Xiao, Ruan Huibing, Hu Xi, Cao Anyi, Song Li
Department of Stomatology, The Second Affiliated Hospital of Nanchang UniversityNanchang, Jiangxi, China.
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Nanchang UniversityNanchang, Jiangxi, China.
Am J Cancer Res. 2017 Apr 1;7(4):913-922. eCollection 2017.
Mutiple microRNAs are implicated in oral squamous cell carcinoma (OSCC), which is characterized by a high rate of proliferation and nodal metastasis. Data from the present study showed that miR-381-3p is significantly underexpressed in both OSCC tissues and cell lines. Overexpression of miR-381-3p led to marked suppression of proliferation and cell cycle progression of OSCC cells and promotion of apoptosis. Notably, fibroblast growth factor receptor 2 (FGFR2) was downregulated by miR-381-3p through direct interactions with its 3' untranslated region. Knockdown of FGFR2 recapitulated the growth suppressive effect of miR-381-3p. Conversely, restoring FGFR2 expression attenuated miR-381-3p-induced effects in OSCC cells. Expression patterns of miR-381-3p and FGFR2 were inversely correlated in OSCC tissues. Our collective results provide novel evidence that miR-381-3p acts as a tumor suppressor in OSCC by directly targeting FGFR2, thereby presenting a promising therapeutic target.
多种微小RNA与口腔鳞状细胞癌（OSCC）有关，其特征是增殖率高和淋巴结转移率高。本研究数据表明，miR-381-3p在OSCC组织和细胞系中均显著低表达。miR-381-3p的过表达导致OSCC细胞的增殖和细胞周期进程受到明显抑制，并促进细胞凋亡。值得注意的是，成纤维细胞生长因子受体2（FGFR2）通过与其3'非翻译区直接相互作用而被miR-381-3p下调。敲低FGFR2可重现miR-381-3p的生长抑制作用。相反，恢复FGFR2表达可减弱miR-381-3p在OSCC细胞中诱导的效应。在OSCC组织中，miR-381-3p和FGFR2的表达模式呈负相关。我们的综合结果提供了新的证据，表明miR-381-3p通过直接靶向FGFR2在OSCC中发挥肿瘤抑制作用，从而呈现出一个有前景的治疗靶点。
