Ophthalmology Department, University of Ottawa, Ottawa, ON, Canada.
Ophthalmology Department, University of British Columbia, Vancouver, BC, Canada.
Int Ophthalmol. 2024 Mar 15;44(1):139. doi: 10.1007/s10792-024-03066-0.
Endothelial cell loss (ECL) during Descemet membrane endothelial keratoplasty (DMEK) graft preparation has been shown to affect graft survival and the need for re-grafting. The purpose of this study was to quantitatively assess the impact of the plastic and glass mediums in contact with DMEK donor tissue during intra-operative graft staining on ECL.
Retrospective study that included patients who underwent DMEK surgery between January 2019 and June 2021 at Hôpital Maisonneuve-Rosemont and the Jewish General Hospital in Montreal, Canada. DMEK grafts were stained with 0.06% Trypan blue ophthalmic solution (VisionBlue®, Dutch Ophthalmic, USA, Exeter, NH) for 120 s in either a plastic or glass medium prior to delivery into the recipient's eye. The ECL was compared between the two groups 12-30 months post-operatively.
ECL at 12-30 months was significantly less in the eyes that had received grafts stained in a plastic medium compared to those stained in a glass medium. Graft survival and re-bubbling was higher in the glass group however this difference was not statistically significant.
Staining of the DMEK graft in a plastic medium caused less ECL compared to the glass medium.
在进行 Descemet 膜内皮角膜移植术（DMEK）供体准备时，内皮细胞丢失（ECL）已被证明会影响移植物的存活率和再次移植的需要。本研究的目的是定量评估术中在塑料和玻璃介质中对 DMEK 供体组织进行染色对 ECL 的影响。
这是一项回顾性研究，纳入了 2019 年 1 月至 2021 年 6 月期间在加拿大蒙特利尔的 Maisonneuve-Rosemont 医院和犹太综合医院接受 DMEK 手术的患者。DMEK 移植物在进入受体眼之前，在塑料或玻璃介质中用 0.06%的锥虫蓝眼科溶液（VisionBlue®，美国荷兰眼科公司，新罕布什尔州埃克塞特）染色 120 秒。在术后 12-30 个月时比较两组之间的 ECL。
在术后 12-30 个月时，在塑料介质中染色的移植物与在玻璃介质中染色的移植物相比，ECL 明显减少。然而，玻璃组的移植物存活率和重新气泡率更高，但这一差异没有统计学意义。
与玻璃介质相比，DMEK 移植物在塑料介质中染色导致的 ECL 减少。
