Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Protein J. 2024 Jun;43(3):503-512. doi: 10.1007/s10930-024-10185-1. Epub 2024 Mar 15.
Metallothioneins are a group of cysteine-rich proteins that play an important role in the homeostasis and detoxification of heavy metals. The objective of this research was to explore the significance of metallothionein in Trichoderma harzianum tolerance to zinc. At the inhibitory concentration of 1000 ppm, the fungus adsorbed 16.7 ± 0.4 mg/g of metal. The HPLC and SDS-PAGE electrophoresis data suggested that the fungus production of metallothionein was twice as high in the presence of zinc as in the control group. The examination of the genes; metallothionein expression activator (MEA) and Cu fist revealed that the MEA, with a CH zinc finger domain, increased significantly in the presence of zinc. It was observed that in T. harzianum, the enhanced expression of the metallothionein gene was managed by the metallothionein activator under zinc overload conditions. According to our knowledge, this is the first report on the role of metallothionein in the resistance of T. harzianum to zinc.
金属硫蛋白是一组富含半胱氨酸的蛋白质，在重金属的体内平衡和解毒中起着重要作用。本研究的目的是探讨金属硫蛋白在哈茨木霉耐受锌中的意义。在 1000ppm 的抑制浓度下，真菌吸附了 16.7±0.4mg/g 的金属。HPLC 和 SDS-PAGE 电泳数据表明，在锌存在的情况下，真菌金属硫蛋白的产量是对照组的两倍。对基因；金属硫蛋白表达激活剂（MEA）和 Cu fist 的检测表明，锌存在时，MEA 的 CH 锌指结构域显著增加。观察到在哈茨木霉中，在锌过载条件下，金属硫蛋白基因的增强表达是由金属硫蛋白激活剂管理的。据我们所知，这是关于金属硫蛋白在哈茨木霉抵抗锌中的作用的第一个报告。
