The Second Hospital of Jilin University, Department of Otolaryngology- Head and Neck Surgery, Changchun, China.
The Second Hospital of Jilin University, Department of Otolaryngology- Head and Neck Surgery, Changchun, China.
Braz J Otorhinolaryngol. 2024 May-Jun;90(3):101410. doi: 10.1016/j.bjorl.2024.101410. Epub 2024 Mar 1.
Our aim in this study is to identify the core genes of chronic rhinosinusitis with nasal polyps and analyze the correlations between it and inflammation-related genes.
GSE72713 dataset containing gene expression data of ECRSwNP, nonECRSwNP and healthy samples was obtained from Gene Expression Omnibus (GEO) and filtered by limma to identify DEGs among three groups, then the functions and correlated pathways of DEGs were analyzed using GO and KEGG. The core DEGs were selected by the intersection of DEGs and the PPI network was constructed via STRING. The correlations between the expression levels of CRSwNP core gene and inflammation-related genes were analyzed via the Mann-Whitney U test.
The DEGs among ECRSwNP, nonECRSwNP, and CTRL were filtered respectively, and enrichment analysis showed they were associated with olfaction and/or immune responses. The PPI network was constructed by 7 core DEGs obtained via the intersection among three groups, and ALOX15 was confirmed as the core gene in the network. Subsequently, the correlations between the expression levels of ALOX15 and inflammation-related genes were illustrated.
In this study, the core gene ALOX15 was selected from the DEGs among ECRSwNP, nonECRSwNP, and CTRL. IL5, IL1RL1, and IL1RAP were found to exhibit a significant positive correlation with ALOX15.
Level 3.
本研究旨在鉴定鼻息肉慢性鼻窦炎的核心基因，并分析其与炎症相关基因的相关性。
从基因表达综合数据库（GEO）中获取包含 ECRSwNP、非 ECRSwNP 和健康样本基因表达数据的 GSE72713 数据集，并通过 limma 进行过滤，以鉴定三组间的差异表达基因（DEGs），然后使用 GO 和 KEGG 分析 DEGs 的功能和相关途径。通过 STRING 构建 PPI 网络，选取 DEGs 的交集作为核心 DEGs。通过 Mann-Whitney U 检验分析 CRSwNP 核心基因表达水平与炎症相关基因之间的相关性。
分别筛选 ECRSwNP、非 ECRSwNP 和 CTRL 间的 DEGs，富集分析显示它们与嗅觉和/或免疫反应相关。通过三组间的交集获得 7 个核心 DEGs 构建 PPI 网络，其中 ALOX15 被确认为网络中的核心基因。随后，阐明了 ALOX15 表达水平与炎症相关基因之间的相关性。
在这项研究中，从 ECRSwNP、非 ECRSwNP 和 CTRL 间的 DEGs 中选择了核心基因 ALOX15。发现 IL5、IL1RL1 和 IL1RAP 与 ALOX15 呈显著正相关。
3 级。
