Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
Brain Struct Funct. 2024 May;229(4):947-957. doi: 10.1007/s00429-024-02769-5. Epub 2024 Mar 18.
The present study aimed to investigate the combination effects of hypothermia (HT) and intranasal insulin (INS) on structural changes of the hippocampus and cognitive impairments in the traumatic brain injury (TBI) rat model. The rats were divided randomly into the following five groups (n = 10): Sham, TBI, TBI with HT treatment for 3 h (TBI + HT), TBI with INS (ten microliters of insulin) treatment daily for 7 days (TBI + INS), and TBI with combining HT and INS (TBI + HT + INS). At the end of the 7th day, the open field and the Morris water maze tests were done for evaluation of anxiety-like behavior and memory performance. Then, after sacrificing, the brain was removed for stereological study. TBI led to an increase in the total volume of hippocampal subfields CA1 and DG and a decrease in the total number of neurons and non-neuronal cells in both sub-regions, which was associated with anxiety-like behavior and memory impairment. Although, the combination of HT and INS prevented the increased hippocampal volume and cell loss and improved behavioral performances in the TBI group. Our study suggests that the combined treatment of HT and INS could prevent increased hippocampal volume and cell loss in CA1 and DG sub-regions and consequently improve anxiety-like behaviors and memory impairment following TBI.
本研究旨在探讨低温（HT）和鼻内胰岛素（INS）联合治疗对创伤性脑损伤（TBI）大鼠模型海马结构变化和认知障碍的影响。大鼠随机分为以下五组（n=10）：假手术组、TBI 组、TBI 后 3 小时 HT 治疗组（TBI+HT）、TBI 后每日 INS 治疗组（TBI+INS）和 TBI 后 HT 和 INS 联合治疗组（TBI+HT+INS）。第 7 天结束时，进行旷场和 Morris 水迷宫测试，以评估焦虑样行为和记忆表现。然后，在牺牲后，取出大脑进行立体学研究。TBI 导致海马 CA1 和 DG 亚区的总体积增加和两个亚区的神经元和非神经元细胞总数减少，与焦虑样行为和记忆损伤有关。尽管 HT 和 INS 的联合治疗可预防 TBI 组海马体积增加和细胞丢失，并改善行为表现。我们的研究表明，HT 和 INS 的联合治疗可预防 CA1 和 DG 亚区海马体积增加和细胞丢失，并改善 TBI 后的焦虑样行为和记忆损伤。
