Department of Biomedical Sciences, Texas A&M University School of Dentistry, 3302 Gaston Ave, Dallas, TX, 75246, USA.
Sci Rep. 2024 Mar 18;14(1):6518. doi: 10.1038/s41598-024-57007-z.
Family with sequence similarity 20, member A (FAM20A) is a pseudo-kinase in the secretory pathway and is essential for enamel formation in humans. Here we examine if FAM20A is a membrane-associated protein. We show that the full-length FAM20A can be purified from HEK293 cells transfected with a FAM20A-expresing construct. Further, it is only found in the membrane fraction, but not in the soluble fraction, of cell lysate. Consistently, it is not secreted out of the expressing cells. Moreover, it is co-localized with GM130, a cis-Golgi network marker, and membrane topology analysis indicates that it has its C-terminus oriented towards the lumen of the organelle. Our results support that FAM20A is a Type II transmembrane protein within the secretory compartments.
家族性 20 号序列相似性成员 A（FAM20A）是分泌途径中的一种拟激酶，对人类牙釉质的形成至关重要。在这里，我们研究 FAM20A 是否是一种膜相关蛋白。我们表明，全长 FAM20A 可以从用表达 FAM20A 的构建体转染的 HEK293 细胞中纯化出来。此外，它只存在于细胞裂解物的膜部分，而不存在于可溶部分。一致地，它不会从表达细胞中分泌出来。此外，它与顺式高尔基体网络标志物 GM130 共定位，并且膜拓扑分析表明它的 C 末端朝向细胞器的腔。我们的结果支持 FAM20A 是分泌隔室中的一种 II 型跨膜蛋白。
