Kikegawa Tatsuki, Yamaguchi Takuya, Nambu Ryohei, Etchuya Kenji, Ikeda Masami, Mukai Yuri
a Department of Electronics, Graduate School of Science and Technology , Meiji University , Kanagawa , Japan.
b Molecular Neurobiology Research Group , Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Ibaraki , Japan.
Biosci Biotechnol Biochem. 2018 Oct;82(10):1708-1714. doi: 10.1080/09168451.2018.1484272. Epub 2018 Jun 18.
Despite studies of the mechanism underlying the intracellular localization of membrane proteins, the specific mechanisms by which each membrane protein localizes to the endoplasmic reticulum, Golgi apparatus, and plasma membrane in the secretory pathway are unclear. In this study, a discriminant analysis of endoplasmic reticulum, Golgi apparatus and plasma membrane-localized type II membrane proteins was performed using a position-specific scoring matrix derived from the amino acid propensity of the sequences around signal-anchors. The possibility that the sequence around the signal-anchor is a factor for identifying each localization group was evaluated. The discrimination accuracy between the Golgi apparatus and plasma membrane-localized type II membrane proteins was as high as 90%, indicating that, in addition to other factors, the sequence around signal-anchor is an essential component of the selection mechanism for the Golgi and plasma membrane localization. These results may improve the use of membrane proteins for drug delivery and therapeutic applications.
尽管对膜蛋白细胞内定位的潜在机制进行了研究，但在分泌途径中，每种膜蛋白定位于内质网、高尔基体和质膜的具体机制仍不清楚。在本研究中，利用从信号锚周围序列的氨基酸倾向推导而来的位置特异性评分矩阵，对定位于内质网、高尔基体和质膜的II型膜蛋白进行了判别分析。评估了信号锚周围序列作为识别每个定位组的一个因素的可能性。高尔基体和质膜定位的II型膜蛋白之间的判别准确率高达90%，这表明除其他因素外，信号锚周围的序列是高尔基体和质膜定位选择机制的一个重要组成部分。这些结果可能会改善膜蛋白在药物递送和治疗应用中的使用。
