State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, PR China.
University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Chem Biodivers. 2024 May;21(5):e202400414. doi: 10.1002/cbdv.202400414. Epub 2024 Apr 18.
Three undescribed sesquiterpenes (1-3), two enantiomeric pairs of monoterpenes (4a/4b-5a/5b), one alkyne (6), two known alkynes (7-8) and eight known coumarins (9-16) were isolated from the aerial parts extracts of Artemisia scoparia. The structures of these compounds were fully elucidated by their 1D and 2D NMR, HRESIMS spectral data analyses, and comparison with literature. The absolute configurations of compounds were determined by single-crystal X-ray crystallography (1), a comparison of experimental and calculated electronic circular dichroism (ECD) data (2-6). 15 showed moderate inhibitory activity with the NO release in LPS-induced RAW264.7 cells. 9-16 showed varying degrees of promoting melanogenesis and tyrosinase activity in B16 cells.
从青蒿地上部分提取物中分离得到三种未描述的倍半萜（1-3）、两种对映体单萜（4a/4b-5a/5b）、一种炔烃（6）、两种已知炔烃（7-8）和八种已知香豆素（9-16）。通过 1D 和 2D NMR、HRESIMS 光谱数据分析以及与文献比较，全面阐明了这些化合物的结构。通过单晶 X 射线晶体学（1）、实验和计算电子圆二色性（ECD）数据（2-6）的比较，确定了化合物的绝对构型。15 对 LPS 诱导的 RAW264.7 细胞中 NO 释放表现出中等抑制活性。9-16 在 B16 细胞中显示出不同程度的促进黑色素生成和酪氨酸酶活性。
