获取人羊膜组织及鉴定多能性标志物。
Obtaining Tissues of Human Amniotic Membrane and Identification of Pluripotent Markers.
机构信息
Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología Isidro Espinosa de Los Reyes, Mexico City, Mexico.
Departamento de Inmunobioquímica, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
出版信息
Methods Mol Biol. 2024;2781:163-170. doi: 10.1007/978-1-0716-3746-3_15.
The immunofluorescence technique has been used to identify pluripotent markers in the human amniotic epithelial cells (hAEC). hAEC belonging to human fetal membranes, specificamently to amnion layer, and are arising by epiblast, this sugest that the hAEC have characteristics of epiblast cells, in other words, characteristcs of pluripotent stem cells. Here we describe obtaining human amnion tissue and identifying pluripotent markers by immunofluorescence.
免疫荧光技术已被用于鉴定人羊膜上皮细胞（hAEC）中的多能性标记物。hAEC 属于人类胎膜，具体来说是羊膜层，由外胚层产生，这表明 hAEC 具有外胚层细胞的特征，换句话说，具有多能干细胞的特征。在这里，我们描述了获得人羊膜组织和通过免疫荧光鉴定多能性标记物的方法。
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