Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Department of Anesthesia, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Immun Inflamm Dis. 2024 Mar;12(3):e1220. doi: 10.1002/iid3.1220.
To investigate the effect of therapeutic hypercapnia on the expression and function of gamma delta T (γδ T) cells during ischemia-reperfusion injury (IRI) after lung transplantation.
We randomly divided male Wistar rats into three groups (n = 6 in each group), the control group (group N), the IRI group (group I), and the therapeutic hypercapnia group (group H). We then assessed pulmonary edema, neutrophil infiltration, wet-to-dry (W/D) weight ratio, and microscopic histopathology and separately measured the levels of γδT cell surface antigen (TCR) and Interleukin-17 (IL-17) using flow cytometry and enzyme-linked immunosorbent assays (ELISAs).
The infiltration of neutrophils and the expression of TCR and IL-17 were significantly increased in the I group compared to the control, and the biopsy edema in group I was more severe. Arterial partial pressure of oxygen (PaO2) was decreased after reperfusion in group I compared with the control group. W/D weight ratio, neutrophil infiltration, and the expression of TCR and IL-17 decreased drastically in the H group compared to the I group.
Our findings suggest that γδ T lymphocytes were directly involved in lung injury. In addition, therapeutic hypercapnia effectively reduced the expression of γδ T cells and IL-17, and this has the potential to become a treatment strategy for IRI and an intervention to improve lung function.
探讨治疗性高碳酸血症对肺移植后缺血再灌注损伤（IRI）期间γδ T（γδ T）细胞表达和功能的影响。
我们将雄性 Wistar 大鼠随机分为三组（每组 6 只），分别为对照组（N 组）、IRI 组（I 组）和治疗性高碳酸血症组（H 组）。然后评估肺水肿、中性粒细胞浸润、湿干重（W/D）比和显微镜组织病理学，并分别使用流式细胞术和酶联免疫吸附试验（ELISA）测量γδ T 细胞表面抗原（TCR）和白细胞介素 17（IL-17）的水平。
与对照组相比，I 组的中性粒细胞浸润和 TCR 和 IL-17 的表达显著增加，且 I 组的活检水肿更严重。与对照组相比，I 组再灌注后动脉血氧分压（PaO2）降低。与 I 组相比，H 组的 W/D 重量比、中性粒细胞浸润以及 TCR 和 IL-17 的表达明显降低。
我们的研究结果表明，γδ T 淋巴细胞直接参与了肺损伤。此外，治疗性高碳酸血症可有效降低 γδ T 细胞和 IL-17 的表达，这可能成为 IRI 的治疗策略，并改善肺功能的干预措施。
