Department of Environmental Engineering, School of Resources and Civil Engineering, Northeastern University, Shenyang, 110819, China.
Department of Environmental Engineering, School of Resources and Civil Engineering, Northeastern University, Shenyang, 110819, China.
Environ Res. 2024 Jun 15;251(Pt 2):118677. doi: 10.1016/j.envres.2024.118677. Epub 2024 Mar 18.
Recent studies demonstrated that plastic degradation in Zophobas atratus superworms is related to the gut microbiota. To determine whether the biodegradation and gut-microbiota were influenced by ingested plastic polymerization types, foams of polypropylene (PP), polyurethane (PU) and ethylene vinyl acetate (EVA) were selected as representatives of polyolefins, polyester and copolymers, and the sole feedstock for superworms for 45 d. Both growth and survival rates of superworms were influenced by the type of plastic diet. Although the total consumptions of EVA- and PP-fed groups were similar at 29.03 ± 0.93 and 28.89 ± 1.14 mg/g-larva, which were both significantly higher than that of PU-fed groups (21.63 ± 2.18 mg/g-larva), the final survival rates of the EVA-fed group of 36.67 ± 10.41% exhibited significantly lower than that of the PP- and PU-fed groups of 76.67 ± 2.89% and 75.00 ± 7.07%, respectively, and even the starvation group of 51.67 ± 10.93%. The Illumina MiSeq results revealed similarities in the dominant gut bacterial communities between PU- and EVA-fed groups, with an increase in relative abundance of Lactococcus, but significant differences from the PP-fed groups, which had two predominant genera of unclassified Enterobacteriaceae and Enterococcus. Compared to bran-fed groups, changes in gut fungal communities were similar across all plastics-fed groups, with an increase in the dominant abundance of Rhodotorula. The abundance of Rhodotorula increased in the order of polyolefin, polyester, and copolymer. In summary, plastic ingestion, larval growth, and changes in gut bacterial and fungal community of superworms were all influenced by foam diets of different polymerization types, and especially influences on the gut microbiomes were different from each other.
最近的研究表明，黄粉虫肠道微生物群与塑料的降解有关。为了确定摄入的塑料聚合类型是否影响生物降解和肠道微生物群，选择了聚丙烯（PP）、聚氨酯（PU）和乙烯-醋酸乙烯酯（EVA）泡沫作为聚烯烃、聚酯和共聚物的代表，以及黄粉虫的唯一饲料，喂养 45 天。不同类型的塑料饮食影响黄粉虫的生长和存活率。尽管 EVA 和 PP 喂养组的总消耗量相似，分别为 29.03±0.93 和 28.89±1.14mg/g-幼虫，但均显著高于 PU 喂养组（21.63±2.18mg/g-幼虫），EVA 喂养组的最终存活率为 36.67±10.41%，显著低于 PP 和 PU 喂养组的 76.67±2.89%和 75.00±7.07%，甚至低于饥饿组的 51.67±10.93%。Illumina MiSeq 结果显示，PU 和 EVA 喂养组的肠道细菌群落具有相似的优势菌，乳球菌的相对丰度增加，但与 PP 喂养组有显著差异，PP 喂养组的主要菌属为未分类肠杆菌科和肠球菌。与麸皮喂养组相比，所有塑料喂养组的肠道真菌群落变化相似，优势菌属根霉菌的丰度增加。根霉菌的丰度按聚烯烃、聚酯和共聚物的顺序增加。综上所述，塑料摄入、幼虫生长以及肠道细菌和真菌群落的变化均受不同聚合类型泡沫饮食的影响，特别是对肠道微生物组的影响彼此不同。
