Matiz-González J Manuel, Ballesteros-Ballesteros Jesús A, Hernández María, Mejorano-Fonseca Julián A, Cuervo Claudia, Faccini-Martínez Álvaro A, Hidalgo Marylin, Pérez-Torres Jairo, Silva-Ramos Carlos Ramiro
Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia.
Zoonoses Public Health. 2024 Aug;71(5):457-468. doi: 10.1111/zph.13126. Epub 2024 Mar 20.
Bats are a diverse group of mammals that have unique features allowing them to act as reservoir hosts for several zoonotic pathogens such as Leptospira. Leptospires have been classified into pathogenic, intermediate, and saprophytic groups and more recently into clades P1, P2, S1, and S2, being all the most important pathogenic species related to leptospirosis included within the P1/pathogenic clade. Leptospira has been detected from bats in several regions worldwide; however, the diversity of leptospires harboured by bats is still unknown.
The aim of the present study was to determine the genetic diversity of Leptospira spp. harboured by bats worldwide.
A systematic review was conducted on four databases to retrieve studies in which Leptospira was detected from bats. All studies were screened to retrieve all available Leptospira spp. 16S rRNA sequences from the GenBank database and data regarding their origin. Sequences obtained were compared with each other and reference sequences of Leptospira species and analysed through phylogenetic analysis.
A total of 418 Leptospira spp. 16S rRNA sequences isolated from 55 bat species from 14 countries were retrieved from 15 selected manuscripts. From these, 417 sequences clustered within the P1/pathogenic group, and only one sequence clustered within the P2/intermediate group. Six major clades of P1/pathogenic Leptospira spp. were identified, three of them composed exclusively of sequences obtained from bats.
We identified that bats harbour a great genetic diversity of Leptospira spp. that form part of the P1/pathogenic clade, some of which are closely related to leptospirosis-associated species. This finding contributes to the knowledge of the diversity of leptospires hosted by bats worldwide and reinforces the role of bats as reservoirs of P1/pathogenic Leptospira spp.
蝙蝠是一类多样的哺乳动物,具有独特特征,使其能够充当多种人畜共患病原体(如钩端螺旋体)的宿主。钩端螺旋体已被分为致病型、中间型和腐生型,最近又被分为进化枝P1、P2、S1和S2,与钩端螺旋体病相关的所有最重要的致病物种都包含在P1/致病进化枝内。在世界多个地区的蝙蝠中都检测到了钩端螺旋体;然而,蝙蝠所携带的钩端螺旋体的多样性仍然未知。
本研究的目的是确定全球蝙蝠所携带的钩端螺旋体属的遗传多样性。
对四个数据库进行系统综述,以检索从蝙蝠中检测到钩端螺旋体的研究。对所有研究进行筛选,以从GenBank数据库中检索所有可用的钩端螺旋体属16S rRNA序列及其来源数据。将获得的序列相互比较,并与钩端螺旋体物种的参考序列进行比较,然后通过系统发育分析进行分析。
从15篇选定的手稿中检索到总共418条从14个国家的55种蝙蝠中分离出的钩端螺旋体属16S rRNA序列。其中,417条序列聚集在P1/致病组内,只有一条序列聚集在P2/中间组内。确定了P1/致病钩端螺旋体属的六个主要进化枝,其中三个完全由从蝙蝠获得的序列组成。
我们发现蝙蝠携带的钩端螺旋体属具有丰富的遗传多样性,这些钩端螺旋体属于P1/致病进化枝的一部分,其中一些与钩端螺旋体病相关物种密切相关。这一发现有助于了解全球蝙蝠所携带的钩端螺旋体的多样性,并加强了蝙蝠作为P1/致病钩端螺旋体属宿主的作用。