Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Biomed Chromatogr. 2024 Jul;38(7):e5857. doi: 10.1002/bmc.5857. Epub 2024 Mar 20.
Palladium-catalyzed coupling reactions are versatile and powerful tools for the construction of carbon-carbon bonds in organic synthesis. Although these reactions have favorable features that proceed selectively in mild reaction conditions using aqueous organic solvents, no attention has been given to their application in the field of biomedical analysis. Therefore, we focused on these reactions and evaluated the scope and limitations of their analytical performance. In this review, we describe the pros and cons and future trends of fluorescence derivatization of pharmaceuticals and biomolecules based on palladium-catalyzed coupling reactions such as Suzuki-Miyaura coupling, Mizoroki-Heck coupling, and Sonogashira coupling reactions for HPLC analysis.
钯催化偶联反应是有机合成中构建碳-碳键的多功能、强大工具。尽管这些反应具有有利的特点，即在温和的反应条件下使用水有机溶剂选择性进行，但它们在生物医学分析领域的应用尚未得到关注。因此，我们专注于这些反应，并评估了它们在分析性能方面的应用范围和局限性。在这篇综述中，我们描述了基于钯催化偶联反应（如 Suzuki-Miyaura 偶联、Mizoroki-Heck 偶联和 Sonogashira 偶联反应）的药物和生物分子的荧光衍生化在 HPLC 分析中的优缺点和未来趋势。
