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绵羊血浆中发情周期和早期妊娠期间循环oar-miR-485-5p和oar-miR-493-5p的表达

Expression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma.

作者信息

Ucar Eyyup Hakan, Hitit Mustafa, Kose Mehmet, Atli Mehmet Osman

机构信息

Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkey.

Department of Animal Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey.

出版信息

Anim Reprod. 2024 Mar 4;21(1):e20230115. doi: 10.1590/1984-3143-AR2023-0115. eCollection 2024.

DOI:10.1590/1984-3143-AR2023-0115
PMID:38510567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10954234/
Abstract

In the current study, we aimed to assess the expression levels of two circulating microRNAs (miRNA) (oar-miR-485-5p and oar-miR-493-5p) in the ovine plasma during the peri-implantation. After mating, we collected the plasma samples from a total of 8 ewes on day 22 of pregnancy (P22; n = 4) and day 22 of the estrous cycle (C22; n=4). We used mature miRNA sequences for oar-miR-485-5p and oar-miR-493-5p out of one hundred fifty, which were retrieved from our microarray results of previous study. We showed that the miRNA expression of oar-miR-485-5p and oar-miR-493-5p were upregulated in P22 (P<0.05) when compared to C22. Those two miRNAs targeted 311 target genes in the peri-implantation period of pregnancy. Furthermore, we revealed 151 GO/pathway terms in biological process (BP) and 25 GO/pathway terms in molecular function (MF), while we demonstrated 13 GO/pathway terms in cellular component (CC). We revealed three hub genes as interleukin 2 (IL2), interleukin 18 (IL18), and C-X-C Motif Chemokine Ligand 10 (CXCL10). In conclusion, both miR-485-5p and oar-miR-493-5p have the potential to be a biomarker to understand peri-implantation of the ovine pregnancy in the aspect of pregnancy-reflected changes in maternal plasma.

摘要

在本研究中，我们旨在评估围植入期绵羊血浆中两种循环微RNA（miRNA）（oar-miR-485-5p和oar-miR-493-5p）的表达水平。交配后，我们在妊娠第22天（P22；n = 4）和发情周期第22天（C22；n = 4）从总共8只母羊中采集了血浆样本。我们从先前研究的微阵列结果中检索出150个中的oar-miR-485-5p和oar-miR-493-5p的成熟miRNA序列。我们发现，与C22相比，P22中oar-miR-485-5p和oar-miR-493-5p的miRNA表达上调（P<0.05）。这两种miRNA在妊娠围植入期靶向311个靶基因。此外，我们在生物学过程（BP）中揭示了151个GO/通路术语，在分子功能（MF）中揭示了25个GO/通路术语，同时在细胞成分（CC）中展示了13个GO/通路术语。我们发现三个枢纽基因，即白细胞介素2（IL2）、白细胞介素18（IL18）和C-X-C基序趋化因子配体10（CXCL10）。总之，miR-485-5p和oar-miR-493-5p都有潜力成为一种生物标志物，从母体血浆中反映妊娠变化的角度来了解绵羊妊娠的围植入期。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/9f172b7843d2/1984-3143-ar-21-1-e20230115-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/b2202872cc06/1984-3143-ar-21-1-e20230115-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/d3cecc76ab07/1984-3143-ar-21-1-e20230115-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/9f172b7843d2/1984-3143-ar-21-1-e20230115-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/b2202872cc06/1984-3143-ar-21-1-e20230115-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/d3cecc76ab07/1984-3143-ar-21-1-e20230115-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b5f/10954234/9f172b7843d2/1984-3143-ar-21-1-e20230115-gf03.jpg

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本文引用的文献

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Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep.
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Front Genet. 2022 Aug 17;13:929477. doi: 10.3389/fgene.2022.929477. eCollection 2022.
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Theriogenology. 2022 Oct 1;191:35-46. doi: 10.1016/j.theriogenology.2022.07.015. Epub 2022 Jul 31.
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