Kabrita Colette S, Al Bitar Samar, Ghanem Esther
Department of Sciences, Faculty of Natural and Applied Sciences, Notre Dame University-Louaize, Zouk Mosbeh, Lebanon.
Brain Behav Immun Health. 2024 Mar 11;37:100751. doi: 10.1016/j.bbih.2024.100751. eCollection 2024 May.
The intricate relationship between sleep and leukocyte trafficking has garnered intense attention, particularly their homing dynamics to secondary lymphoid organs under normal and restricted sleep (SR). Considering the scarcity of information regarding circadian rhythms in major histocompatibility class I (MHC-I) expression in SR, we designed a study that assessed the temporal expression of MHC-I in murine lymph nodes and spleen and the subsequent effects of sleep recovery. Male C57BL/6, housed in 12:12 light/dark cycle, were grouped into control (C) and SR. SR was carried for one week before lymphoid tissues were sampled at selected time points and assessed for leukocyte number and MHC-I expression. SR resulted in 21% decrease in granulocyte and 24% increase in agranulocyte numbers. In C, MHC-I expression pattern in lymph nodes was bimodal and relatively higher than splenocytes during the animal's active phase (110.2 ± 1.8 vs 81.9 ± 3.8, respectively; p = 0.002). Splenocytes; however, showed a bimodal pattern upon SR, with higher protein levels during the rest than the activity period (154.6 + 36.2 vs 99.5 + 15.9, respectively; p = 0.002), suggesting preparedness for a potential infection. Furthermore, SR caused a significant drop in MHC-I expression at the onset of rest with 57% and 30% reduction in lymph nodes and splenocytes, respectively. However, the overall protein expression collectively taken from both lymphoid tissues remained stable, emphasizing its indispensable role in immunological homeostasis. This stability coincided with the restoration of protein levels to baseline after a short sleep recovery period, resembling a reset for MHC-I antigen presentation following a week of SR. Understanding the interplay between MHC-I expression and contextual factors could enhance treatment protocols, refining the efficacy and time precision of glucocorticoid-based therapies in immune modulation.
睡眠与白细胞运输之间的复杂关系已引起广泛关注，尤其是在正常睡眠和受限睡眠（SR）情况下它们向次级淋巴器官的归巢动态。鉴于关于SR中主要组织相容性复合体I类（MHC-I）表达的昼夜节律信息匮乏，我们设计了一项研究，评估MHC-I在小鼠淋巴结和脾脏中的时间表达以及睡眠恢复的后续影响。将饲养在12:12光/暗周期中的雄性C57BL/6小鼠分为对照组（C）和SR组。在选定时间点对淋巴组织进行采样并评估白细胞数量和MHC-I表达之前，先进行一周的SR。SR导致粒细胞减少21%，无粒细胞数量增加24%。在C组中，淋巴结中MHC-I的表达模式呈双峰，在动物活动期相对高于脾细胞（分别为110.2±1.8和81.9±3.8；p=0.002）。然而，脾细胞在SR时呈现双峰模式，休息期的蛋白质水平高于活动期（分别为154.6 + 36.2和99.5 + 15.9；p=0.002），表明对潜在感染有所准备。此外，SR在休息开始时导致MHC-I表达显著下降，淋巴结和脾细胞分别减少57%和30%。然而，从两个淋巴组织总体获取的蛋白质表达保持稳定，强调了其在免疫稳态中的不可或缺作用。这种稳定性与短时间睡眠恢复期后蛋白质水平恢复到基线相吻合，类似于一周SR后MHC-I抗原呈递的重置。了解MHC-I表达与相关因素之间的相互作用可以改进治疗方案，提高基于糖皮质激素的免疫调节疗法的疗效和时间精度。
