Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, CSIC. Profesor Albareda, 1. Granada 18008, Spain.
Laboratory Affiliated to Istituto Pasteur Italia - Fondazione Cenci Bolognetti - Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Rome, Italy.
Microbiology (Reading). 2024 Mar;170(3). doi: 10.1099/mic.0.001449.
Different bacteria change their life styles in response to specific amino acids. In (now ) KT2440, arginine acts both as an environmental and a metabolic indicator that modulates the turnover of the intracellular second messenger c-di-GMP, and expression of biofilm-related genes. The transcriptional regulator ArgR, belonging to the AraC/XylS family, is key for the physiological reprogramming in response to arginine, as it controls transport and metabolism of the amino acid. To further expand our knowledge on the roles of ArgR, a global transcriptomic analysis of KT2440 and a null mutant growing in the presence of arginine was carried out. Results indicate that this transcriptional regulator influences a variety of cellular functions beyond arginine metabolism and transport, thus widening its regulatory role. ArgR acts as positive or negative modulator of the expression of several metabolic routes and transport systems, respiratory chain and stress response elements, as well as biofilm-related functions. The partial overlap between the ArgR regulon and those corresponding to the global regulators RoxR and ANR is also discussed.
不同的细菌会根据特定的氨基酸改变它们的生活方式。在（现在的）KT2440 中，精氨酸既是环境又是代谢指示剂，调节细胞内第二信使 c-di-GMP 的周转率，并调节生物膜相关基因的表达。属于 AraC/XylS 家族的转录调节因子 ArgR 是响应精氨酸进行生理重编程的关键，因为它控制着氨基酸的运输和代谢。为了进一步扩展我们对 ArgR 作用的认识，对 KT2440 和在精氨酸存在下生长的缺失突变体进行了全局转录组分析。结果表明，这种转录调节因子影响了多种细胞功能，超出了精氨酸代谢和运输的范围，从而扩大了其调节作用。ArgR 作为几种代谢途径和运输系统、呼吸链和应激反应元件以及生物膜相关功能的表达的正或负调节剂。ArgR 调节子与全局调节因子 RoxR 和 ANR 的调节子之间也存在部分重叠。
